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ARS Home » Southeast Area » Stoneville, Mississippi » Genomics and Bioinformatics Research » Research » Publications at this Location » Publication #353496

Title: A Spinach Genome for the Next Generation of Breeders

item Hulse-Kemp, Amanda
item BOSTAN, HAMED - North Carolina State University
item ASHRAFI, HAMID - North Carolina State University
item STOFFEL, KEVIN - University Of California, Davis
item SANSEVERINO, WALTER - Sequentia Biotech Sl
item LI, LINZHOU - Bgi Shenzhen
item CHENG, HIFENG - Bgi Shenzhen
item TSENG, ELIZABETH - Pacific Biosciences Inc
item CHIN, JASON - Pacific Biosciences Inc
item IORIZZO, MASSIMO - North Carolina State University
item VAN DEYNZE, ALLEN - University Of California, Davis

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/17/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Spinach is a leafy vegetable which is a rich source of vitamins and micronutrients which has become an increasingly important part of many diets as shown by the increased world-wide production over the past couple decades. Next generation sequencing technologies have exponentially increased the number of species for which genome sequence is available. While technologies with long-read sequencing has become increasingly utilized to develop high-quality genome sequences, most publicly available genome sequences are still predominately in draft stage composed of a high-number of contigs and many of which are not anchored into chromosome scale assemblies. Here we report the completion of the first publicly available spinach genome using long-read sequencing. Sequencing-based maps generated from a genetic mapping population were utilized along with mate pair sequencing to generate pseudomolecules in which the N80 falls into the six spinach chromosomes. Annotation produced 34,878 gene models of which 1,004 are annotated as resistance genes. A highly contiguous and anchored genome will allow for enhanced capabilities of future studies for the next generation of spinach breeders.