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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #351457

Research Project: Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm

Location: National Germplasm Resources Laboratory

Title: Advances in virus diagnostics

item Hammond, John
item Jordan, Ramon
item Mollov, Dimitre

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/12/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Plant viruses are among the most difficult pathogens to identify in the typical plant diagnostic laboratory. In this seminar, diagnosticians will learn the strengths and weaknesses of various detection methods. An overview of virus diagnostics will be presented: ELISA, including different formats, broad-spectrum and high-specificity assays, and lateral flow assays (immunostrips); PCR/RT-PCR and related assays; next generation sequencing and analysis. Case studies of hard-to-diagnose viral diseases (such as Rose rosette virus) will be presented. Opportunities to utilize the latest technologies in a range of diagnostic laboratories will be highlighted.