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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #348964

Research Project: Discovery and Introgression of Disease Resistance Genes into Phaseolus vulgaris

Location: Soybean Genomics & Improvement Laboratory

Title: Sequencing the complex genome of Uromyces appendiculatus, the bean rust pathogen

Author
item Hurtado-Gonzales, Oscar
item DIAZ-VALDERRAMA, JORGE - Purdue University
item AIME, CATHERINE - Purdue University
item Pastor Corrales, Marcial - Talo

Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Trade Journal
Publication Acceptance Date: 6/5/2018
Publication Date: 6/5/2018
Citation: Hurtado-Gonzales, O.P., Diaz-Valderrama, J.R., Aime, C.M., Pastor Corrales, M.A. 2018. Sequencing the complex genome of Uromyces appendiculatus, the bean rust pathogen. Bean Improvement Cooperative Annual Report. 61:63-64.

Interpretive Summary:

Technical Abstract: The extensive virulence diversity of Uromyces appendiculatus segregates into two distinct groups corresponding to the Mesoamerican and Andean gene pools of common bean. The similarities between the virulence and genetic diversities of the bean rust pathogen and the common bean respectively, suggest that these organisms have undergone a parallel co-evolution. The objective of the present study was to use genomics to find new opportunities to improve our understanding of the interaction between common bean and the bean rust pathogen and of the complex diversity of this pathogen. We sequenced one Andean (5-0) and one Mesomerican race (31-1) of the bean rust pathogen. A total of 73.4 Gb and 69.6 Gb of data were generated for races 5-0 and 31-1, respectively. Genome assemblies of the two races resulted in a high number of contigs, suggesting a complex, and highly repetitive genome, that is larger (>500Mb) than most of the reported genomes of fungal plant pathogens. A total of 110,000 SSRs were identified and a subset of 16 SSRs were tested on 46 races of U. appendiculatus. A genetic-distance analysis of these 16 SSRs, separated the 46 races into two different groups of races, one Andean and another Mesoamerican. This separation suggests that the bean rust pathogen has co-evolved with its common bean host. These results are providing insights into the evolution of the races of U. appendiculatus. These results could also be used for the surveillance of new races and may provide information leading to the identification of virulence genes in U. appendiculatus.