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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #348835

Research Project: Genetic Enhancement of Watermelon, Broccoli, and Leafy Brassicas for Economically Important Traits

Location: Vegetable Research

Title: QTL-seq analysis of heat tolerance in broccoli

item Branham, Sandra
item Farnham, Mark

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 12/18/2017
Publication Date: 1/15/2018
Citation: Branham, S., Farnham, M.W. 2018. QTL-seq analysis of heat tolerance in broccoli. Plant and Animal Genome Conference Proceedings. p.0568.

Interpretive Summary:

Technical Abstract: Broccoli (Brassica oleracea var. italica) production, worth approximately a billion dollars annually in the United States alone, is restricted in terms of location and season, due to the sensitivity of commercial cultivars to high temperatures. Heat stress during heading causes yield and quality loss of the crop. Several heat tolerant broccoli lines have been developed in recent years but few studies have examined the underlying genetic basis. Three years of summer field trials were used to evaluate heat tolerance in a doubled haploid population of broccoli. Bulked segregant analysis was combined with whole genome resequencing for a QTL-seq study of heat tolerance. Two QTL were identified and collocated with several strong candidate genes, including those encoding heat shock proteins and antioxidants.