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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Publications at this Location » Publication #348694

Research Project: Biology, Epidemiology and Management of Vector-Borne Viruses of Sugarbeet and Vegetable Crops

Location: Crop Improvement and Protection Research

Title: Deciphering how BNYVV overcomes Rhizomania resistance genes in sugarbeet through metabolome analysis

Author
item Wintermantel, William - Bill
item Broeckling, Corey - Colorado State University
item Richardson, Kelley
item Webb, Kimberly

Submitted to: International Congress of Plant Pathology Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/27/2018
Publication Date: 7/29/2018
Citation: Wintermantel, W.M., Broeckling, C., Richardson, K.L., Webb, K.M. 2018. Deciphering how BNYVV overcomes Rhizomania resistance genes in sugarbeet through metabolome analysis. International Congress of Plant Pathology, July 29-August 3, 2018, Boston, Massachusetts.

Interpretive Summary: Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), is one of the most important diseases of sugarbeet and is distributed in most growing areas of the world. BNYVV can be controlled through single resistance genes. However, BNYVV strains with the ability to overcome the widely used Rz1 gene have been emerging in many production areas. To fully understand how sugarbeet responds to BNYVV we have utilized a proteomic and metabolomic approach to identify the biological pathways associated with Rhizomania resistance. Earlier proteomic studies demonstrated that a relatively small number of proteins were associated with BNYVV infection and resistance in sugarbeet. In this work, we utilized a metabolomics approach to further characterize the functional pathways associated with BNYVV sugarbeet lines that were genetically uniform, but contained either the Rz1 or Rz2 resistance genes for control of rhizomania disease. Plants were grown for three weeks in growth chambers in either healthy soil, soil with traditional BNYVV, or soil containing Rz1 resistance breaking BNYVV (RB-BNYVV). Metabolites were extracted from roots and evaluated by mass spectrometry. Results identified significant differences among treatments, with primary metabolomic differences associated with strain of BNYVV as compared to healthy (non-infected) sugarbeet. These studies build on the knowledge generated through previous proteomic research, and may lead to identification of targets for genetic modification to break the virus-host interaction.

Technical Abstract: Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), is one of the most important diseases of sugarbeet and is distributed in most growing areas of the world. BNYVV can be controlled through single resistance genes. However, BNYVV strains with the ability to overcome the widely used Rz1 gene have been emerging in many production areas. To more fully understand how sugarbeet responds to BNYVV we have utilized a proteomic and metabolomic approach to identify the biological pathways associated with Rhizomania resistance. Earlier proteomic studies demonstrated that a relatively small number of proteins were associated with BNYVV infection and resistance in sugarbeet. We utilized a metabolomics approach to further characterize the functional pathways associated with BNYVV in near isogenic lines containing either the Rz1 or Rz2 resistance gene. Plants were grown for three weeks in growth chambers in either healthy soil, soil with traditional BNYVV, or soil containing Rz1 resistance breaking BNYVV (RB-BNYVV). Metabolites were extracted from roots and evaluated by GC-MS and UPLC-MS. There were significant differences among treatments, with primary metabolomic differences associated with strain of BNYVV as compared to healthy (non-infected) sugarbeet. These studies build on the knowledge generated through previous proteomic research, and may lead to identification of targets for genetic modification to break the virus-host interaction.