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Research Project: Genetic Analysis of Complex Traits in Maize

Location: Plant Science Research

Title: Large scale field inoculation and scoring of maize southern leaf blight and other maize foliar fungal diseases

Author
item Sermons, Shannon
item Balint-Kurti, Peter

Submitted to: Bio-protocol
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/2018
Publication Date: 5/1/2018
Citation: Sermons, S.M., Balint Kurti, P.J. 2018. Large scale field inoculation and scoring of maize southern leaf blight and other maize foliar fungal diseases. Bio-protocol. https://doi:10.21769/BioProtoc.2745.
DOI: https://doi.org/10.21769/BioProtoc.2745

Interpretive Summary: This manuscript describes a protocol for large scale inoculation and scoring of maize Southern Leaf Blight disease in the field. It also describes slight modifications for inoculating and scoring the other maize diseases, Gray Leaf Spot and Northern Leaf Blight.

Technical Abstract: Field-grown maize is inoculated with Cochliobolus heterostrophus, causal agent of Southern Leaf Blight disease, by dropping sorghum grains infested with the fungus into the whorl of each maize plant at an early stage of growth. The initial lesions produce secondary inoculum that is dispersed by wind and rain, causing multiple cycles of infection that assures a high uniform disease pressure over the entire field by the time of disease scoring, which occurs after anthesis. This method, with slight modifications, can also be used to study the maize fungal diseases Northern Leaf Blight (caused by Exserohilum turcicum) and Gray Leaf Spot (Cercospora zeae-maydis).