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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #347065

Research Project: Analysis of Virulence and Antibiotic Resistance Mechanisms of Salmonella and Development of Intervention Strategies

Location: Food Safety and Enteric Pathogens Research

Title: Modulation of porcine microRNAs associated with apoptosis and NF-kappaB signaling pathways in response to Salmonella enterica serovar Typhimurium

Author
item Shippy, Daniel
item Bearson, Bradley - Brad
item Cai, Guohong
item Brunelle, Brian
item KICH, JALUSA - Embrapa-Pigs And Poultry
item Bearson, Shawn

Submitted to: Gene
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/8/2018
Publication Date: 11/15/2018
Publication URL: http://handle.nal.usda.gov/10113/6132692
Citation: Shippy, D.C., Bearson, B.L., Cai, G., Brunelle, B.W., Kich, J., Bearson, S.M. 2018. Modulation of porcine microRNAs associated with apoptosis and NF-kappaB signaling pathways in response to Salmonella enterica serovar Typhimurium. Gene. 676:290-297. https://doi.org/10.1016/j.gene.2018.08.044.
DOI: https://doi.org/10.1016/j.gene.2018.08.044

Interpretive Summary: Salmonella enterica serovar Typhimurium is a human foodborne pathogen which causes significant economic loss worldwide. Pigs are a major source of salmonellosis in humans, as Salmonella can be transmitted from pigs to humans through consumption of contaminated pork products and direct contact with infected pigs. To reduce the burden of Salmonella to the pork industry, a better understanding of the regulatory mechanisms of the swine response to Salmonella would be beneficial. In this study, the swine response to Salmonella was evaluated by measuring the expression of pig genes, and a group of gene expression regulators (microRNAs), from the whole blood of pigs over a 7-day period. Following inoculation with Salmonella, pigs displayed a significant change in gene and microRNA expression, specifically those involved in the swine immune response and programmed cell death (apoptosis). Overall, the results of our study identified new candidate microRNAs in the swine response to Salmonella, and further defined regulatory factors to target for improving food safety.

Technical Abstract: MicroRNAs (miRNAs) are small, non-coding RNAs that regulate eukaryotic gene expression at the post-transcriptional level. In addition to their involvement in a variety of biological processes, miRNAs are implicated in the eukaryotic response to bacterial pathogens. The objective of this study was to identify miRNAs involved in the regulation of the porcine response to the human foodborne pathogen, Salmonella enterica serovar Typhimurium (S. Typhimurium). Differential expression analysis from the whole blood of pigs over a 7-day period following S. Typhimurium challenge identified 50 miRNAs, many of which are implicated in functional pathways associated with NF-kappaB signaling and apoptosis (e.g., ssc-let-7c, ssc-miR-21). Transcriptome analyses of whole blood mRNA identified the differential expression of several genes involved in NF-kappaB signaling and apoptosis (e.g., IL10, CBX4, TGFB2) whose mRNA are predicted targets of miRNAs identified in our study. Overall, our data identified porcine miRNAs that are differentially expressed following S. Typhimurium challenge, thereby defining regulatory factors to target for controlling the porcine response to this human foodborne pathogen.