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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Emerging Pests and Pathogens Research » Research » Publications at this Location » Publication #345688

Research Project: Characterization of Molecular Networks in Diseases Caused by Emerging and Persistent Bacterial Plant Pathogens

Location: Emerging Pests and Pathogens Research

Title: Transcriptomic profiling suggests that promysalin alters metabolic flux, motility, and iron regulation in Pseudomonas putida KT2440

Author
item Giglio, Krista - Former ARS Employee
item Keohane, Colleen - Emory University
item Stodghill, Paul
item Steele, Andrew - Emory University
item Fetzer, Christian - Technische Universitat Munchen
item Sieber, Stephen - Technische Universitat Munchen
item Filiatrault, Melanie
item Wuest, William - Emory University

Submitted to: ACS Infectious Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/25/2018
Publication Date: 5/25/2018
Citation: Giglio, K., Keohane, C.E., Stodghill, P., Steele, A.D., Fetzer, C., Sieber, S., Filiatrault, M.J., Wuest, W.M. 2018. Transcriptomic profiling suggests that promysalin alters metabolic flux, motility, and iron regulation in Pseudomonas putida KT2440. ACS Infectious Diseases. https://doi.org/10.1021/acsinfecdis.8b00041.
DOI: https://doi.org/10.1021/acsinfecdis.8b00041

Interpretive Summary: Promysalin was recently discovered as a natural product produced by the bacterium Pseudomonas putida strain RW10S1. The natural product is able to inhibit growth of some bacteria, and promote surface motility in other bacteria. To determine the mechanism of how the compound promotes surface motility we grew bacteria in the presence and absence of the compound and analyzed global changes in gene expression. We found that the genes involved in motility as well as iron acquisition and storage are generally down regulated in bacterial cells treated with the compound. Our data also indicate that treatment with the compound induces a shift in bacterial metabolism. Overall, these data provide insight in the mechanism of action of the compound produced by Pseudomonas putida and suggests cells treated with promysalin experience iron-limited growth conditions.

Technical Abstract: Promysalin, a secondary metabolite produced by P. putida RW10S1, is a narrow-spectrum antibiotic that targets P. aeruginosa over other Pseudomonas spp. P. putida KT2440, a non-producing strain, displays increased swarming motility and decreased pyoverdine production in the presence of exogenous promysalin. Herein, proteomic and transcriptomic experiments were used to provide insight about how promysalin elicits responses in PPKT2440 and rationalize its species-selectivity. RNA-sequencing results suggest that promysalin affects PPKT2440 by 1) increasing swarming in a flagella-independent manner; 2) causing cells to behave as if they were experiencing an iron-deficient environment, and 3) shifting metabolism away from glucose conversion to pyruvate via the Entner- Doudoroff pathway. These findings highlight Nature’s ability to develop small molecules with specific targets that result in exquisite selectivity.