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ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Mosquito and Fly Research » Research » Publications at this Location » Publication #341023

Research Project: Biting Arthropod Surveillance and Control

Location: Mosquito and Fly Research

Title: Preparative Capillary GC for Characterization of Five Dracocephalum Essential Oils from Mongolia, and their Mosquito Larvicidal Activity

Author
item Ozek, Gulmira - ANADOLU UNIVERSTIY
item Tabanca, Nurhayat
item Radwan, Mohammes - UNIVERSITY OF MISSISSIPPI
item Shatar, Sanduin - MONGOLIAN ACADEMY OF SCIENCES
item Altantsetseg, Altaa - MONGOLIAN ACADEMY OF SCIENCES
item Baatar, Dumaajav - MONGOLIAN ACADEMY OF SCIENCES
item Basar, Kemal - ANADOLU UNIVERSTIY
item Becnel, James
item Ozek, Temel - ANADOLU UNIVERSTIY

Submitted to: Natural Product Communications
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/29/2016
Publication Date: 6/1/2016
Citation: Ozek, G., Tabanca, N., Radwan, M.M., Shatar, S., Altantsetseg, A., Baatar, D., Basar, K.H., Becnel, J.J., Ozek, T. 2016. Preparative Capillary GC for Characterization of Five Dracocephalum Essential Oils from Mongolia, and their Mosquito Larvicidal Activity. Natural Product Communications. Vol. 11 (10) 2016 pp 1541-1544.

Interpretive Summary: Because of increasing insecticide resistance, new pesticides are needed. Natural products have long been a fruitful source to identify new environmentally friendly pesticides. In this study, 5 essential oils from 5 species of the perennial plant Dracocephalum were evaluated for activity against the yellow fever mosquito Aedes aegypti. Results from the current study found larvicidal activity but no adulticidal activity for Ae. aegypti with several of the essential oils. This is the first study to provide a detailed chemical composition and mosquitocidal activity of essential oils from Mongolian Dracocephalum species.

Technical Abstract: (DF), D. moldavica L. (DM), D. fruticulosum Steph. ex Willd. (DFr) and D. peregrinum L. (DP) were evaluated. Simultaneous GC-FID and GC-MS analyses revealed in the EOs of DR, DF and DM an unidentified compound (1) ([M+122], 5.4%, 57.9% and 74.0%, respectively). Therefore, we aimed to isolate compound 1 using Preparative Capillary GC (PCGC) connected to a Preparative Fraction Collector (PFC) system. Structure determination of 1 was determined by 1H- and 13C-NMR as p-mentha-1,8-dien-10-al (1, limonen-10-al). Other detected major constituents were thymol (34.0%) and carvacrol (6.1%) in DR; limonene (28.8%) in DF; cis-chrysanthenol acetate (29.1%) and trans-verbenol (5.0%) in DFr; and linalool (17.9%), trans-'-bergamotene (7.9%), (E)- nerolidol (7.7%) and eugenol (5.5%) in DP. The DFr, DP, DR and DM EOs produced 100% mortality to 1st instar larvae of Aedes aegypti L. at 250 ppm and DR and DM EO exhibited the strongest activity and killed 100% 1st instar larvae at 62.5 ppm. No adulticidal activity was observed against female Ae. aegypti. of the present study, for the first time isolation of limonen-10-al (1) from DR, DM, and DP EOs and larvicidal activity of five Dracocephalum Os were reported.