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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #340816
Research Project: Umbrella Project for Food Safety

Location: Warmwater Aquaculture Research Unit

Title: Supplemental invasion of Salmonella from the perspective of Salmonella enterica serovars Kentucky and Typhimurium

Author
item HOWE, KEVIN - Mississippi State University
item SALEHI, SANAZ - Mississippi State University
item BAILEY, H - Mississippi State University
item Brooks, John
item WILLS, R - Mississippi State University
item LAWRENCE, MARK - Mississippi State University
item KARSI, ATTILA - Mississippi State University

Submitted to: BMC Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/22/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary: Type III secretion system (T3SS) is an important virulence factor responsible for bacterial invasion. In this study, we aimed to understand the role of T3SS in Salmonella enterica serovar Kentucky and Typhimurium. Results indicated that Kentucky was able to invade human colorectal adenocarcinoma epithelial Caco-2 cells, which was significantly lower compared to Typhimurium. Additionally, T3SS mutants were less invasive than the wild-type strains, but the decrease in invasion was not significant in Kentucky.

Technical Abstract: Background: Critical to the development of Salmonellosis in humans is the interaction of the bacterium with the epithelial lining of the gastrointestinal tract. Traditional scientific reasoning held type III secretion system (T3SS) as the virulence factor responsible for bacterial invasion. In this study, field-isolated Salmonella enterica serovar Kentucky and a known human pathogen Salmonella enterica serovar Typhimurium were mutated and evaluated for the invasion of human colorectal adenocarcinoma epithelial cells. Results: S. enterica serovar Kentucky was shown to actively invade a eukaryotic monolayer, though at a rate that was significantly lower than Typhimurium. Additionally, strains mutated for T3SS formation were less invasive than the wild-type strains, but the decrease in invasion was not significant in Kentucky. Conclusions: Strains mutated for T3SS formation were able to initiate invasion of the eukaryotic monolayer to varying degrees based on strain, In the case of Kentucky, the mutated strain initiated invasion at a level that was not significantly different from the wild-type strain. A different result was observed for Typhimurium as the mutation significantly lowered the rate of invasion in comparison to the wild-type strain.