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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #339826

Research Project: Genetics, Breeding and Reproductive Physiology to Enhance Production of Catfish

Location: Warmwater Aquaculture Research Unit

Title: Complete genome sequence of Edwardsiella ictaluri isolate RUSVM-1 recovered from nile tilapia (Oreochromis niloticus) in the Western Hemisphere

Author
item Reichley, Stephen - Mississippi State University
item Waldbieser, Geoffrey - Geoff
item Soto, Estaban - University Of California
item Lawrence, Mark - Mississippi State University
item Griffin, Matt - Mississippi State University

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/4/2017
Publication Date: 6/15/2017
Citation: Reichley, S.R., Waldbieser, G.C., Soto, E., Lawrence, M.L., Griffin, M.J. 2017. Complete genome sequence of Edwardsiella ictaluri isolate RUSVM-1 recovered from nile tilapia (Oreochromis niloticus) in the Western Hemisphere. Genome Announcements. 5:e00390-17.

Interpretive Summary: Catfish infected with Edwardsiella ictaluri develop a systemic septicemia that leads to significant production losses on US catfish farms. E. ictaluri strain RUSVM-1 is pathogenic to tilapia and zebrafish but it is serologically and genetically divergent from the typical E. ictaluri isolates associated with farm-raised catfish. The biological implications of the genetic differences, the threat RUSVM-1 may pose to the catfish industry, are currently unknown. We have sequenced the genome of RUSVM-1 in order to support research to identify the specific genetic differences between this and other E. ictaluri strains. The RUSVM-1 genome will be useful for comparisons analyses with other E. ictaluri strains to identify differential gene content, gene expression patterns, and protein products that influence pathogenicity, and could serve as targets for vaccines or therapeutants.

Technical Abstract: Edwardsiella ictaluri is a Gram-negative, bacillus that has recently been implicated in disease outbreaks in tilapia and zebrafish. We report here the complete and annotated genome of an isolate from a Nile Tilapia (Oreochromis niloticus), which contains a chromosome of 3,630,639 bp and two plasmids.