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ARS Home » Southeast Area » Fayetteville, Arkansas » Poultry Production and Product Safety Research » Research » Publications at this Location » Publication #339610

Research Project: Antibiotic Alternatives for Controlling Foodborne Pathogens and Disease in Poultry

Location: Poultry Production and Product Safety Research

Title: Antimicrobial wash with Trans-cinnamaldehyde nanoemulsion reduces Campylobacter jejuni on chicken skin

item UPADHYAY, ABHINAV - University Of Arkansas
item WAGLE, BASANTA - University Of Arkansas
item SHRESTHA, SANDIP - University Of Arkansas
item UPADHYAYA, INDU - University Of Arkansas
item ARSI, KOMALA - University Of Arkansas
item BHARGAVA, KANIKA - University Of Arkansas
item Donoghue, Ann - Annie
item DONOGHUE, DAN - University Of Arkansas

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Campylobacter jejuni is a major foodborne pathogen that causes severe enteritis in humans largely due to consumption of contaminated poultry products. Reducing C. jejuni contamination on chicken carcasses would reduce subsequent human infections. This study investigated the efficacy of Trans-cinnamaldehyde (TC; generally recognized as safe status antimicrobial from cinnamon bark) as a wash treatment for reducing C. jejuni on chicken skin. In addition, the effect of TC treatments on chicken skin color was analyzed using Chroma meter (CR 400/410, Konica Minolta, NJ, USA). Chicken skin samples (5 cm x 5 cm) were spot inoculated with a 4-strain cocktail of C. jejuni (approximately 6 log CFU/sample) followed by attachment time of 30 min at room temperature. Subsequently, the inoculated skin samples were treated with 0.5, 1% TC treatments (normal or nanoemulsion form) either with or without application of shaking for 1, 3, and 5 min. The treated samples were drip dried for 2 min and the number of surviving C. jejuni on chicken skin were enumerated. Each treatment had 5 samples and the study was repeated two times. The data were analyzed using ANOVA with GraphPad Prism ver. 6. Differences between the means were considered significantly different at P<0.05. TC 0.5 and 1% dip treatments did not significantly reduce C. jejuni counts on chicken skin even after 5 min of dipping time. However, the nanoemulsion form of TC at 0.5 and 1% significantly reduced C. jejuni counts by approximately 1.8 log and 2 log CFU/ml after 1 min of dipping treatment (P<0.05). Increasing the contact time of TC nanoemulsions from 1 to 3 or 5 min did not further reduce the counts of C. jejuni on skin (P>0.05). Shaking of skin samples in antimicrobial treatments enhanced the antimicrobial efficacy of both normal and nanoemulsion form of TC. By 5 min of treatment time, both normal and nanoemulsion form of TC at 0.5 and 1% reduced C. jejuni counts by at least 2.6 and 2.7 log CFU/ml respectively. There was no change in the color of skin treated with TC (P>0.05). Results suggest that TC (normal and nanoemulsion forms) can be used as antimicrobial wash treatments for reducing C. jejuni survival on chicken skin. Follow up analysis testing the efficacy of TC on chicken wingettes is currently underway.