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ARS Home » Southeast Area » Fayetteville, Arkansas » Poultry Production and Product Safety Research » Research » Publications at this Location » Publication #339609

Research Project: Antibiotic Alternatives for Controlling Foodborne Pathogens and Disease in Poultry

Location: Poultry Production and Product Safety Research

Title: In-water supplementation of Trans-cinnamaldehyde nanoemulsion reduces Campylobacter jejuni colonization in broiler chickens

item UPADHYAY, ABHINAN - University Of Arkansas
item ARSI, KOMALA - University Of Arkansas
item WAGLE, BASANTA - University Of Arkansas
item UPADHYAYA, INDU - University Of Arkansas
item SHRESTHA, SANDIP - University Of Arkansas
item BHARGAVA, KANIKA - University Of Arkansas
item Donoghue, Ann - Annie
item DONOGHUE, DAN - University Of Arkansas

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/17/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Campylobacter jejuni is a major foodborne pathogen that causes severe gastroenteritis in humans. Chickens act as the reservoir host for C. jejuni, wherein the pathogen colonizes the ceca thereby leading to contamination of the carcass during slaughter. Reducing C. jejuni cecal colonization could potentially reduce the risk of human infections. This study investigated the efficacy of in-water supplementation of Trans-cinnamaldehyde (TC; generally recognized as safe status compound from Cinnamon bark) nanoemulsion in reducing C. jejuni cecal colonization in 14-day-old broiler chickens. In addition, the effect of TC on colonization factors (motility, attachment to chicken enterocytes) was investigated using a motility bioassay and cell culture analysis. In two separate trials, day of hatch broiler chickens (Cobb 500; 10 birds/treatment/trial) were supplemented with TC (normal or nanoemulsion form) in drinking water at 0, 0.0625, 0.125, 0.25, 0.5, and 1% level for 14 days. On day 7, the birds were challenged with a four-strain cocktail of C. jejuni (approximately 6 log CFU/bird) by oral gavage. On day 14, the birds were sacrificed and C. jejuni colonization in cecal contents were quantified. Data were analyzed using ANOVA with GraphPad ver. 6. Differences between the means were considered significantly different at P<0.05. Administration of TC nanoemulsion (Polydispersity index <0.3; size approximately 100-200 nm; zeta potential approxiamately -0.35 mV) in drinking water at 0.25% reduced C. jejuni colonization by approximately 1 or 2 logs CFU/mL in trial 1 or trial 2 as compared to respective controls (P<0.05). Follow up mechanistic analysis revealed that TC reduced pathogen motility and attachment to primary chicken enterocytes (P<0.05). No reduction in feed consumption, water intake or body weight gain was observed in 0.25% or lower concentration treatments as compared to controls (P>0.05). Results suggest that TC nanoemulsion could potentially be used to control C. jejuni colonization in broiler chickens. Follow up analysis on the effect of TC on cecal microbiome and C. jejuni transcriptome and proteome in broiler chickens is currently underway.