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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #339038

Research Project: CHARACTERIZING, DETECTING, AND ELIMINATING PATHOGENS TO ENABLE THE SAFE INTRODUCTION OF PLANT GENETIC RESOURCES

Location: National Germplasm Resources Laboratory

Title: Survey of six rose viruses in a rose virus collection

Author
item Farrar, Kristen - University Of California
item Klaassen, Vicki - University Of California
item Mollov, Dimitre
item Zlesak, David - University Of Wisconsin
item Sim, Sue - University Of California
item Golino, Deborah - University Of California
item Al Rwahnih, Maher - University Of California

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: More than 25 viruses have been reported to infect roses. As part of the routine diagnostic testing at Foundation Plant Services, roses are tested for viruses by biological, serological, and molecular assays. Over the past 18 years, we identified approximately 600 roses that were worth maintaining as a collection for further evaluation based on industry needs. None of these roses qualify as foundation material. The phytosanitary status of this rose virus collection was evaluated over the past two years. In a preliminary study to determine the optimal tissue to use for testing, leaves, petioles, and stems were compared as templates for RNA quality and quantity using an automated extraction (Kingfisher) protocol. Extracts from leaves yielded the most concentrated and purest total RNA, and leaves are the most abundant and easiest to collect rose material. The rose virus collection was tested by RT-qPCR for Apple mosaic virus (ApMV), Prunus necrotic ringspot virus (PNRSV), Rose spring dwarf-associated virus (RSDaV), Rose rosette virus (RRV), Rose yellow vein virus (RYVV), and Blackberry chlorotic ringspot virus (BCRV). Up to 254 samples of roses were tested singly or as composite samples of the same genotype. Testing was conducted biannually to consider potential titer differences between spring and fall. Some seasonal differences were observed in the first year and testing for the second year is underway. Results indicate that the rose virus collection is heavily infected with ApMV, PNRSV, and RSDaV. RYVV was also detected in the virus collection. This was the first report of RYVV in California. No roses tested positive for RRV or BCRV.