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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #338416

Research Project: Developing Soybean and Other Legumes with Resistance to Pathogens and Assessing the Biosafety of Transgenic Soybean

Location: Soybean Genomics & Improvement Laboratory

Title: An overview of soybean seed protein analysis

Author
item MARIA JOHN, K - Us Forest Service (FS)
item Luthria, Devanand - Dave
item Natarajan, Savithiry - Savi

Submitted to: Journal of the Science of Food and Agriculture
Publication Type: Review Article
Publication Acceptance Date: 5/10/2018
Publication Date: 7/3/2018
Citation: Maria John, K.M., Luthria, D.L., Natarajan, S.S. 2018. An overview of soybean seed protein analysis. Journal of the Science of Food and Agriculture. 98:5572-5580.

Interpretive Summary: New and improved soybeans are made through a combination of classical breeding and biotechnology. New varieties are required to meet the global demand for soybeans. To ensure safety and nutritional quality, there is a critical need to investigate the changes in protein content of the new varieties and their food products. One method for doing this is called mass spectrometry. Mass spectrometry can identify and measure the amounts of proteins found in a sample. Such analysis begins with an optimal combination of extraction, separation, and detection of proteins. In this review article, we have attempted to provide a brief summary of current updates in the methodologies used for extraction, separation, and detection of proteins extracted from soybeans. This information can be effectively used by scientists at government agenicies, universities and private corporations who investigate the changes in protein contents in new varieties of soybeans and other crops.

Technical Abstract: Development of improved soybean cultivars through classical breeding and new biotech approaches are important to meet global demand for soybeans. To ensure the safety and nutritional quality of the new soybean varieties and their food products, there is a critical need to investigate changes in protein content and profiles. A proteomics study begins with an optimal combination of extraction, separation, and detection approaches. In this review, we have attempted to provide a brief summary of current updates in the methodologies used for extraction, separation, and detection of protein extracted from soybean. This information can be effectively used to investigate the changes in protein content and profiles in new varieties of soybeans and other crops.