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ARS Home » Southeast Area » Fayetteville, Arkansas » Poultry Production and Product Safety Research » Research » Publications at this Location » Publication #338246

Research Project: Antibiotic Alternatives for Controlling Foodborne Pathogens and Disease in Poultry

Location: Poultry Production and Product Safety Research

Title: Protein profiles of hatchery egg shell membrane

item Rath, Narayan
item LIYANAGE, ROHANA - University Of Arkansas
item MAKKAR, SARBJEET - University Of Arkansas
item LAY, JACK - University Of Arkansas

Submitted to: Proteome Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/21/2017
Publication Date: 3/3/2017
Citation: Rath, N.C., Liyanage, R., Makkar, S., Lay, J. 2017. Protein profiles of hatchery egg shell membrane. Proteome Science. 15:4.

Interpretive Summary: Hatchery egg shell membranes (HESM) as feed supplement have shown potential to improve immunity of post hatch chickens and protect against endotoxin stress. We hypothesized that it may partly be due to the protein constituents of the membrane. To understand, we analyzed HESM using mass spectrometry which can identify constituent proteins and peptides and found that that it contained many autogenic proteins that regulate different cellular and biological processes and many proteins of microbial origins. We think that these factors may be consequential for the development of immunity of poultry against pathogens and reducing their stress susceptibility.

Technical Abstract: Background: Eggshells, which consist largely of calcareous outer shell and shell membranes, constitute a significant part of poultry hatchery waste. The shell membranes (ESM) not only contain proteins that originate from egg whites but also from the developing embryos and different contaminants of microbial and environmental origins. As feed supplements, during post hatch growth, the hatchery egg shell membranes (HESM) have shown potential for imparting resistance of chickens to endotoxin stress and exert positive health effects. Considering that these effects are mediated by the bioactive proteins and peptides present in the membrane, the objective of the study was to identify the protein profiles of hatchery eggshell membranes (HESM). Methods: Hatchery egg shell membranes were extracted with acidified methanol and a guanidine hydrochloride buffer then subjected to reduction/alkylation, and trypsin digestion. The methanol extract was additionally analyzed by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). The tryptic digests were analyzed by liquid chromatography and tandem mass spectrometry (LC-MS-MS) to identify the proteins. Results: Our results showed the presence of several proteins that are inherent and abundant in egg white such as, ovalbumin, ovotransferrin, ovocleidin-116, and lysozyme, and several proteins associated with cytoskeletal, cell signaling, antimicrobial, and catalytic functions involving carbohydrate, nucleic acid, and protein metabolisms. There were some blood derived proteins most likely originating from the embryos and several other proteins identified with different aerobic, anaerobic, gram positive, gram negative, soil, and marine bacterial species some commensals and others zoonotic. Conclusion: The variety of bioactive proteins, particularly the cell signaling and enzymatic proteins along with the diverse microbial proteins, make the HESM suitable for nutritional and biological application to improve post hatch immunity of poultry.