|ISANI, GLORIA - University Of Bologna|
|FERLIZZA, ENEA - University Of Bologna|
Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 11/1/2017
Publication Date: 2/12/2018
Citation: Isani, G., Ferlizza, E., Nally, J.E. 2018. Proteomic research in urine and other fluids. Book Chapter. 121-147. https://doi.org/10.1007/978-3-319-69682-9_7.
Interpretive Summary: Book Title: Proteomics in Domestic Animals: from farm to Systems Biology Chapter Title: Proteomic Research in Urine and Other Fluids This book chapter provides a review of the application of the tools of proteomics to the analysis of urine and other fluids (cerebrospinal fluid, bronchoalveolar lavage fluid and amniotic fluid) from a range of farm and companion animal species.
Technical Abstract: Urine is an ideal biological sample. It is an important source of clinical biomarkers of systemic and renal diseases and can be obtained repeatedly without causing stress. Different analytical protocols for the study of the urine proteome have been used, from the simplest electrophoresis on agarose gels to the most sensitive and complex 2DE. As with urine, a range of bodily fluids are amenable to the tools of proteomics. The identification and characterization of the protein content of such fluids are being used to provide novel insights into the evolutionary adaptations of farm and domestic animal species, and to characterize their normal physiological state. A comprehensive review on the application of proteomics to these biological samples is presented.