|SUI, XUELIAN - Fujian Agriculture And Forest University|
|ZHENG, YI - Boyce Thompson Institute|
|TIAN, TONGYAN - California Department Of Food And Agriculture|
|GROTH-HELMS, DEBORAH - Agdia|
|KEINATH, ANTHONY - Clemson University|
|FEI, ZHANGJUN - Boyce Thompson Institute|
|WU, ZUJIAN - Fujian Agriculture And Forest University|
Submitted to: Tomato Disease Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 10/25/2016
Publication Date: 11/1/2016
Citation: Sui, X., Zheng, Y., Li, R., Padmanabhan, C., Tian, T., Groth-Helms, D., Keinath, A.P., Fei, Z., Wu, Z., Ling, K. 2016. Molecular and biological characterization of Tomato mottle mosaic virus and development of reverse transcription-polymerase chain reaction for species-specific detection. Tomato Disease Workshop. Pgs 16-17.
Interpretive Summary: N/A
Technical Abstract: Tomato mottle mosaic virus (ToMMV) was first identified in 2013 as a novel tobamovirus infecting tomatoes in Mexico. In just a few years, ToMMV has been identified in several countries around the world, including the United States. In the present study, we characterized the molecular, serological and biological properties of ToMMV and developed a species-specific reverse transcription-polymerase chain reaction (RT-PCR) to detect three tomato-infecting tobamoviruses: Tobacco mosaic virus (TMV), Tomato mosaic virus (ToMV) and ToMMV. Previously, ToMMV has been reported in Florida and New York. In this study, we made two new reports on the occurrences of ToMMV on tomatoes in California and South Carolina. Their complete genome sequences were revealed and their genetic relationships to other tobamoviruses were evaluated. In host range study, some differential responses in host plants were identified between ToMMV and ToMV. To alleviate the cross-serological reactivity among the three tomato-infecting tobamoviruses, a new multiplex RT-PCR was developed to allow for a species-specific detection and identification of TMV, ToMV and ToMMV. In addition, we observed resistance breaking by ToMMV on selected tomato cultivars that were resistant to ToMV. These unique biological properties of ToMMV have caused serious concerns to the tomato growers worldwide. In conclusion, the knowledge in the molecular and biological properties would provide us fundamental information to manage this emerging virus on tomato and other solanaceous crops in the U.S. and around the world.