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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Cotton Structure and Quality Research » Research » Publications at this Location » Publication #334316
Research Project: Improved Quality Assessments of Cotton from Fiber to Final Products

Location: Cotton Structure and Quality Research

Title: Characterization of developmental immature fiber (im) mutant and Texas Marker-1 (TM-1) cotton fibers by Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) spectroscopy

Author
item Liu, Yongliang
item Kim, Hee-Jin

Submitted to: Applied Spectroscopy
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/18/2016
Publication Date: 1/20/2017
Citation: Liu, Y., Kim, H.J. 2017. Characterization of developmental immature fiber (im) mutant and Texas Marker-1 (TM-1) cotton fibers by Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) spectroscopy. Applied Spectroscopy. 71(7):1689-1695. https://doi.org/10.1177/0003702816684639.
DOI: https://doi.org/10.1177/0003702816684639

Interpretive Summary: The immature fiber (im) mutant is one type of cotton fiber mutants with unique characteristics of non-fluffy cotton bolls. Compared to its near-isogenic wild type Texas Marker-1 (TM-1), im fiber has thin secondary cell wall and is less mature. This study reported the use of ATR-FTIR technique to compare the structural changes during their secondary cell wall biosyntheses between two types of fibers, through principal component analysis (PCA) and previously proposed simple algorithms. The difference between TM-1 and corresponded im fibers was apparently detected, when comparing the normalized intensity variations of both 735 and 708 cm-1 bands. Notably, the 735 cm-1 band intensities in developmental im fibers are consistently lower than those in developmental TM-1 fibers. The outcome provides cotton breeders and quality researchers an effective diagnostic tool in monitoring im gene regulated fiber cellulose biosynthesis for cotton physiology and breeding improvement.

Technical Abstract: The immature fiber (im) mutant is one type of cotton fiber mutants with unique characteristics of non-fluffy cotton bolls. Compared to its near-isogenic wild type Texas Marker-1 (TM-1), im fiber has thin secondary cell wall and is less mature. In this work, we applied the previously proposed principal component analysis (PCA) and simple algorithms to analyze the attenuated total reflection Fourier transform infrared (ATR FT-IR) spectra of developmental im and TM-1 fibers. The results from these approaches could not effectively and consistently indicate the inherent difference between TM-1 and im fibers at same developmental stage. The difference between TM-1 and corresponded im fibers was apparently detected, when comparing the normalized intensity variations of both 735 and 708 cm-1 bands. Notably, the 735 cm-1 band intensities in developmental im fibers are consistently lower than those in developmental TM-1 fibers. The observation might imply the likelihood of containing relatively less amorphous regions in im fibers than in TM-1 fibers, which is potentially related with its immature phenotype.