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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #332383

Research Project: HOST-PATHOGEN INTERACTIONS IN BARLEY AND WHEAT

Location: Cereal Crops Research

Title: Barley 4H QTL confers NFNB resistance to a global set of P. teres f. teres isolates

Author
item Islamovic, Emir
item Bregitzer, Paul
item Friesen, Timothy

Submitted to: Molecular Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/8/2017
Publication Date: 3/6/2017
Citation: Islamovic, E., Bregitzer, P.P., Friesen, T.L. 2017. Barley 4H QTL confers NFNB resistance to a global set of P. teres f. teres isolates. Molecular Breeding. 37:29. doi:10.1007/s11032-017-0621-0.

Interpretive Summary: Pyrenophora teres f. teres is a fungal pathogen that causes net form net blotch (NFNB) on barley worldwide. A segregating population of 132 progeny lines developed from a cross of the barley varieties 'Falcon' and 'Azhul' was used to evaluate resistance to NFNB due to their differential reaction to isolates of P. teres f. teres from Australia, Canada, Japan, and USA. Falcon is a six-rowed, hulless feed barley harboring resistance to NFNB, while Azhul is a six-rowed, hulless food barley with high levels of susceptibility to many P. teres f. teres isolates. Seedling disease resistance data were collected and the population was genotyped using Illumina’s GoldenGate assay and quantitative trait loci were detected on chromosomes 2H, 3H, 4H, and 6H. We identified a single genetic region on barley chromosome 4H that provided varying levels of resistance to all P. teres f. teres isolates evaluated.

Technical Abstract: Net form net blotch (NFNB), caused by Pyrenophora teres f. teres Drechs., is prevalent in barley-growing regions worldwide. A population of 132 recombinant inbred lines (RILs) developed from a cross of the barley varieties 'Falcon' and 'Azhul' were used to evaluate resistance to NFNB due to their differential reaction to isolates of P. teres f. teres from Australia, Canada, Japan, and USA. Falcon is a six-rowed, hulless feed barley harboring resistance to NFNB, while Azhul is a six-rowed, hulless food barley with high levels of susceptibility to many P. teres f. teres isolates. Seedling disease resistance data were collected on seedlings of parents, RILs, and checks in a growth chamber. The population was genotyped using Illumina’s GoldenGate assay and QTL were detected on chromosomes 2H, 3H, 4H, and 6H. We identified a single genetic region on barley chromosome 4H that provided varying levels of resistance to all P. teres f. teres isolates evaluated.