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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #331760

Research Project: INTERVENTION STRATEGIES TO CONTROL VIRAL DISEASES OF CATTLE

Location: Ruminant Diseases and Immunology Research

Title: Variation in pestivirus growth in testicle primary cell culture is more dependent on the individual cell donor than cattle breed

Author
item Weber, Matheus - Universidade Federal Do Rio Grande Do Norte
item Bauermann, Fernando - U.s. Department Of Agriculture (USDA)
item Romero, Ninnet - Universidad Nacional Autonoma De Mexico
item Herring, Andy - Texas A&m University
item Canal, Claudio - Universidade Federal Do Rio Grande Do Norte
item Neill, John
item Ridpath, Julia

Submitted to: Veterinary Research Communications
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/8/2016
Publication Date: 3/1/2017
Citation: Weber, M.N., Bauermann, F.V., Romero, N.E., Herring, A.D., Canal, C.W., Neill, J.D., Ridpath, J.F. 2017. Variation in pestivirus growth in testicle primary cell culture is more dependent on the individual cell donor than cattle breed. Veterinary Research Communications. 41(1):1-7. doi: 10.1007/s11259-016-9666-5.

Interpretive Summary: Domestic cattle belong to two different groups known as subspecies. The two subspecies Bos taurus indicus (indicine cattle) and Bos taurus taurus (taurine cattle). There are differences between the two subspecies. For example, taurine cattle have greater growth rates in the absence of stressful situations, while indicine exhibit higher heat tolerance and resistance to some parasites and pathogens such as bovine tuberculosis. Bovine pestiviruses infect both indicine and taurine cattle and such infections may contribute to the development of respiratory disease. The goal of this study was to compare the growth of different bovine pestiviruses in cell cultures obtained from taurine, indicine and mixed taurine and indicine cattle. Significant differences in virus growth did not correlate with cattle subspecies. However, significant differences were observed between cells derived from different individuals regardless of subspecies. Variation in the replication of virus in primary cell strains may reflect a genetic predisposition that favors virus replication. The model developed in this study would allow comparison of in vitro observations with in vivo health outcomes during beef production.

Technical Abstract: Bovine respiratory disease complex (BRDC) affects cattle from all breeds living in all regions. However, different breeds of cattle appear to have different susceptibilities to developing BRDC. The causes of BRDC are multifactorial and include infection with both viral and bacterial pathogens. Infection with bovine pestiviruses, including bovine viral diarrhea virus 1 (BVDV1), BVDV2 and ‘HoBi’-like viruses, is linked to the development of BRDC. The aim of the present study was to compare the growth of different bovine pestiviruses in primary testicle cell cultures obtained from taurine, indicine and mixed taurine and indicine cattle breeds. Primary cells strains, derived from testicular tissue, were generated from three animals from each breed. Bovine pestivirus strains used were from BVDV-1a, BVDV-1b, BVDV-2a and ‘HoBi’-like virus. Growth was compared by determining virus titers after one passage in primary cells. All tests were run in triplicate. Virus titers were determined by endpoint dilution and RT-qPCR. Statistical analyses was performed using one way analysis of variance (ANOVA) followed by the Tukey’s Multiple Comparison Test (P less than 0.05). Significant differences in virus growth was did not correlate with cattle breed. However, significant differences were observed between cells derived from different individuals regardless of breed. Variation in the replication of virus in primary cell strains may reflect a genetic predisposition that favors virus replication.