|Manikandan, Velu - Chonbuk National University|
|Velmurugan, Palanivel - Chonbuk National University|
|Park, Jung-hee - Chonbuk National University|
|Seo, Sang-ki - Korea Rural Community Corporation|
|Javanthi, Peryasamy - Periyar University|
|Cho, Min - Chonbuk National University|
|Oh, Byung-taek - Chonbuk National University|
Submitted to: Materials Letters
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/25/2016
Publication Date: 8/26/2016
Publication URL: http://handle.nal.usda.gov/10113/5700729
Citation: Manikandan, V., Velmurugan, P., Park, J., Lovanh, N.C., Seo, S., Javanthi, P., Cho, M., Oh, B. 2016. Synthesis and antimicrobial activity of palladium nanoparticles from Prunus × yedoensis leaf extract. Materials Letters. 185:335-338.
Interpretive Summary: The high demand for protein consumption in the ever-increasing population has put great pressure on food animal production system. To increase profit margin along with productivity, the utilization of antibiotics to promote animal growth and reduce mortality has contributed to the emergence of resistant bacteria that may affect humans as well as the animals themselves. The rise in antibiotic resistant bacteria is not due to just the overuse of antibiotics in agricultural settings but also due to the inappropriate use of antibiotics in human medicine as well. Thus it is necessary to find a simple and economical way to counter or reduce the proliferation of these antibiotic resistant bacteria. Nanoparticles with antimicrobial properties hold a great promise in this arena. Among the diverse metallic nanoparticles, palladium nanoparticles (PdNPs) have gained more importance in the scientific community due to their antimicrobial properties as well as their wide applications in medicine, biology, material sciences, physics, chemistry, their unique properties/use as catalysts/electrocatalysts, polymer membranes, sensor design applications, coatings, plastics, nano fiber, textiles, fuel cells, and active membranes. In this study, nontoxic, eco-friendly methods for the synthesis of metallic nanoparticles using plant extracts were carried out along with the study on the antimicrobial properties of palladium nanoparticles obtained from these plant extracts. The results show that PdNPs has a remarkable activity against antibiotic resistant bacteria. Therefore, the present study provides a potential eco-friendly and sustainable way for the synthesis of antimicrobial nanoparticles from plant extracts that may be quite useful in reducing pathogens in agricultural as well as human medicinal settings.
Technical Abstract: The eco-friendly production of palladium nanoparticles (PdNPs) by Prunus × yedoensis tree leaf extract was studied for the first time. Initial confirmation of PdNP production was confirmed by a color change from light yellow to dark brown. The optimization parameters show that pH 7, 8% leaf extract, 1.5 mM metal ion, and a 30 min timeframe are essential for the production of PdNPs. The UV-Visible spectra (421 nm), X-ray diffraction peaks (2'=42.5°), and Fourier transform infrared spectroscopy confirmed the presence of PdNPs and phytoconstituents. Morphological structure analysis using high-resolution transmission electron microscopy shows that the PdNPs are mostly spherical in shape with rough edges with a size of 20-50 nm. Among all the gram-positive and gram-negative bacteria tested, Bacillus subtilis (6 mm) and Pseudomonas aeruginosa (5 mm) showed a zone of inhibition against the synthesized PdNPs at 200 µg/well.