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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #331251
Research Project: Genetics, Breeding and Reproductive Physiology to Enhance Production of Catfish

Location: Warmwater Aquaculture Research Unit

Title: Complete genome sequence of Edwardsiella piscicida isolate S11-285 recovered from channel catfish (Ictalurus punctatus) in Mississippi, USA

Author
item REICHLEY, STEPHEN - Mississippi State University
item Waldbieser, Geoffrey - Geoff
item TEKEDAR, HASAN - Mississippi State University
item LAWRENCE, MARK - Mississippi State University
item GRIFFIN, MATT - Mississippi State University

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/7/2016
Publication Date: 11/23/2016
Citation: Reichley, S.R., Waldbieser, G.C., Tekedar, H.C., Lawrence, M.L., Griffin, M.J. 2016. Complete genome sequence of Edwardsiella piscicida isolate S11-285 recovered from channel catfish (Ictalurus punctatus) in Mississippi, USA. Genome Announcements. 4(6):e01259-16. doi:10.1128/genomeA.01259-16.

Interpretive Summary: The bacteria Edwardsiella tarda had been implicated in fish mortalities based on functional classification methods. Recent DNA analyses have shown the classical E. tarda group is composed of three species - E. tarda, E. anguillarum, and E. piscicida. Of these, E. piscicida is most virulent to channel catfish and increasing recovered from diseased farm-raised catfish in the southeastern U.S. Researchers at the Warmwater Aquaculture Research Unit and Mississippi State University College of Veterinary Medicine in Stoneville, MS, recently produced a complete genome sequence for E. piscicida isolate S11-285. This sequence show 99.5% sequence similarity to another E. piscicida isolate, 94.5% similarity to two E. anguillarum isolates, and only 83% similarity to an E. tarda isolate. It was more similar to the sequence of E. ictaluri, a known catfish pathogen, than to E. tarda. The genomic sequence will permit more accurate diagnosis of Edwardsiella infections in farm-raised catfish and provide a basis for realizing mechanisms of pathogenesis in this group of bacteria.

Technical Abstract: Edwardsiella piscicida is a recently described Gram-negative facultative anaerobe and an important pathogen to many wild and cultured fish species worldwide. Here we report the complete and annotated genome of E. piscicida isolate S11-285 recovered from channel catfish (Ictalurus punctatus) consisting of a chromosome of 3,923,603 bp and 1 plasmid.