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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #329637

Title: Proteomic analysis of anti-nutritional factors (ANF’s) in soybean seeds as affected by environmental and genetic factors

item K.M., MARIAJOHN - Us Forest Service (FS)
item KHAN, FAROOQ - University Of Maryland
item Luthria, Devanand - Dave
item Garrett, Wesley
item Natarajan, Savithiry - Savi

Submitted to: Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/12/2016
Publication Date: 9/19/2016
Citation: K.M., M., Khan, F., Luthria, D.L., Garrett, W.M., Natarajan, S.S. 2016. Proteomic analysis of anti-nutritional factors (ANF’s) in soybean seeds as affected by environmental and genetic factors. Food Chemistry. 218:321-329.

Interpretive Summary: Soybean is a rich and inexpensive source of proteins for humans and animals. But it also contains many compounds which are considered allergenic or anti-nutritional. Different soybean varieties are grown in different geographic locations throughout the United States. These locations have different growing seasons and climates and different soil compositions. To determine if the natural soybean seed protein profiles vary from cultivar to cultivar, from location to location, and from season to season, we characterized soybean anti-nutritional proteins from three states, Maryland, South Carolina and South Dakota. In addition, we compared anti-nutritional proteins across genotypes and environments to observe any relationships. We found significant differences in the expression of proteins with respect to environmental factors/genetic factors. These results are important for the evaluation of soybean seed production and will be of interest to soybean growers, breeders and producers at universities, government agencies and private industry.

Technical Abstract: The genotype (G), environment (E), and the relationship between G and E on soybean seed anti-nutritional factors (ANFs) were examined under three different agro-climatic conditions. The field trials were conducted at Maryland, South Carolina, and South Dakota using nine region specific genotypes. At each location, the nine genotypes were grown with two planting/sowing dates. Differentially expressed protein spots from the two-dimensional gel electrophoresis were analyzed using mass spectrometry. Seven ANFs corresponding to soybean agglutinin and Kunitz trypsin inhibitor were identified based on the statistical significance levels at p<0.005. The G and environmental conditions (planting/sowing season) influences the ANF’s content. Early sowing dates greatly reduce the total ANF’s content irrespective of genotypes and their growing locations.