|MAGGIOLI, MAYARA - Iowa State University|
|VORDEMEIER, H - Animal & Plant Health Agency Apha|
|MCGILL, JODI - Kansas State University|
|LARSEN, MICHELLE - Albert Einstein College Of Medicine|
Submitted to: American Association of Immunologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 4/25/2016
Publication Date: 5/10/2016
Citation: Maggioli, M.F., Palmer, M.V., Thacker, T.C., Vordemeier, H.M., Mcgill, J.L., Larsen, M.H., Waters, W.R. 2016. Polyfunctional cytokine production by central memory T cells from cattle in response to Mycobacterium bovis infection and BCG vaccination. American Association of Immunologists Proceedings. page 33.
Technical Abstract: Polyfunctional T cells simultaneously produce IFN-gamma, IL-2 and TNF-alpha and play relevant roles in several chronic infections, including TB. Mycobacterium bovis infection of cattle elicits ex vivo polyfunctional T cell responses. Vaccine-elicited IFN-gamma Tcm (CD4 plus CD45RO plus CCR7 plus) responses correlate to protection in cattle. The objective was to assess polyfunctional Tcm responses to vaccination with BCG or BCG mutants and subsequent M. bovis challenge in cattle. BCG induced polyfunctional Tcm to PPD and Ag85A/TB10.4. After challenge, TNF-alpha/IFN-gamma Tcm responses to PPD, Ag85A/TB10.4 or ESAT-6:CFP10 were greater in non-vaccinates in comparison to vaccinates; while TNF-alpha/IFN-gamma/IL-2 Tcm responses were lower. Both vaccines showed similar protection; however, vaccinated animals that were M. bovis culture negative had lower TNF-alpha/IFN-gamma polyfunctional Tcm responses to ESAT-6:CFP10 3wpi than did culture positive vaccinates. In conclusion, BCG vaccination elicited Tcm polyfunctionality, mainly TNF-alpha/IFN-gamma co-producers. Dampened TNF-alpha/IFN-gamma and greater TNF-alpha/IFN-gamma/IL-2 Tcm responses after challenge may indicate enhanced pathogen control and correlate with vaccine elicited protection.