Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/16/2016
Publication Date: 7/27/2016
Citation: Filiatrault, M.J., Fishman, M., Stodghill, P. 2016. Probing Pseudomonas syringae host interactions using metatranscriptomics. American Phytopathological Society Annual Meeting. p. 44.
Technical Abstract: Transcriptome analyses during the interaction of plants and pathogens can be used to provide insights into molecular mechanisms of plant resistance as well as the mechanisms used by bacteria to adapt to hosts and cause disease. We performed a dual in planta RNA-Seq experiment to profile RNA expression in both the plant pathogen Pseudomonas syringae and host before and after effector deployment. P. syringae DC3000 deltahopQ1-1 was syringe infiltrated into Nicotiana benthamiana. Analysis of the RNA-Seq data showed that approximately 2000 P. syringae transcripts displayed differential expression between one hour and six hours post inoculation. We found that as disease progressed bacterial transcripts that encode for siderophores and motility were generally down regulated whereas genes involved in coronatine biosynthesis, alginate production, and the utilization of carbon sources such as fructose, sucrose and maltose were upregulated during infection. We observed that most genes related to Type III secretion system (T3SS) are down regulated in planta between one and six hours, however some components of the T3SS are induced, suggesting that there are other factors involved in the regulation of a subset of the T3SS components. Overall our results support the notion that during disease progression, P. syringae displays increased metabolic activity along with decreased movement and differential regulation of several Type III effectors occurs in planta.