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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Publications at this Location » Publication #328305

Research Project: Genetic Enhancement of Lettuce, Spinach, Melon, and Related Species

Location: Crop Improvement and Protection Research

Title: Genetics of the partial resistance against race 2 of Verticillium dahliae in lettuce

Author
item Sandoya, German - University Of California
item Truco, Maria Jose - University Of California
item Bertier, Lien - University Of California
item Subbarao, Krishna - University Of California
item Michelmore, Richard - University Of California
item Simko, Ivan
item Hayes, Ryan

Submitted to: American Society of Horticulture Science Meeting
Publication Type: Proceedings
Publication Acceptance Date: 4/12/2016
Publication Date: 8/8/2016
Citation: Sandoya, G.V., Truco, M., Bertier, L., Subbarao, K.V., Michelmore, R., Simko, I., Hayes, R.J. 2016. Genetics of the partial resistance against race 2 of Verticillium dahliae in lettuce. American Society for Horticultural Science Annual Meeting. Available: https://ashs.confex.com/ashs/2016/webprogram/Paper24442.html.

Interpretive Summary:

Technical Abstract: Lettuce (Lactuca sativa L.) production on the Coastal California is threatened by Verticillium wilt, a soil borne fungal disease caused by Verticillium dahliae that diminishes yield and quality. Two races of V. dahliae were identified on lettuce, race 1 and race 2. Complete resistance to race 1 is conferred by a single dominant gene, but only partial resistance (PR) to race 2 is known. The objective of this research was to determine the inheritance of PR in 99 F3 families derived from a cross between PI 171674 and PI 204707 both of which exhibit PR. Plants were inoculated with race 2 isolate VdLs17 in two greenhouse experiments with three replications. The parents and the susceptible cv. Salinas were replicated 11 times. Disease Severity (DS) was rated on a scale of 0 (no root discoloration) to 5 (complete root discoloration and wilting lower leaves). Disease Incidence (DI) was calculated as the proportion of symptomatic plants. The Percentage of Foliar Wilting (PFW) and Days to First Flower (DFF) were also evaluated for each plant. Families were genotyped using Genotyping-By-Sequencing and SNPs were identified using TASSEL 3.0. A genetic map was constructed using JoinMap and QTL analysis was conducted using Composite Interval Mapping feature of QGene. Both parental accessions (PI 171674, DI = 18% and PI 204707, DI = 52%) had significantly less disease than cv. Salinas (DI = 71%). In the F3 families, DI ranged 4% - 70%. Significant differences among F3 families for DI, DS and DFF were detected, but no families with resistance significantly higher or lower than both parents were found. Three QTLs were identified for DI, DS and PFW on linkage groups (LG) 5, 6, and 8 explaining 4%, 12%, and 6% of the phenotypic variation (R2), respectively. Environmentally specific QTL were detected on LG 3, 4, and 5, demonstrating the occurrence of QTL x environment interactions. DFF had a large effect QTL in LG7 (R2 = 30%) and a smaller QTL in LG4 (R2 = 4%) that were not linked with resistance QTLs. The presence of multiple QTL indicates that there are several genes controlling PR and that resistance is strongly influenced by the environment. Large numbers of progeny need to be screened to identify favorable allele combinations.