|CUNNINGHAM, HANNAH - University Of Wyoming|
|CAMMACK, K - University Of Wyoming|
Submitted to: International Society for Animal Genetics (ISAG)
Publication Type: Abstract Only
Publication Acceptance Date: 5/25/2016
Publication Date: 7/23/2016
Citation: Lindholm-Perry, A.K., Cunningham, H.C., Kuehn, L.A., Keele, J.W., Cammack, K., Freetly, H.C. 2016. Transcriptome profile of genes differentially expressed in the mesenteric adipose tissue of beef cattle with variation in feed intake and gain. International Society for Animal Genetics (35th ISAG). Abstract Book. p. 73-74 (Abstract #P3042).
Technical Abstract: Mesenteric fat, a depot within the visceral fat, is responsible for hydrolyzing fatty acids and glycerol and releases more free fatty acids into the plasma than subcutaneous fat. In humans, visceral fat depots contribute to obesity and obesity-related disorders. The aim of this study was to determine whether the genes expressed in the mesenteric fat of steers were associated with body weight gain and feed intake. Sixteen steers chosen by their rank of distance from the bivariate mean for gain and feed intake were selected for this study. Mesenteric fat was obtained and evaluated for differences in gene expression. A total of 1,831 genes were identified as differentially expressed among steers with variation in feed intake and gain. Many of these genes were involved with metabolic processes such as proteolysis, transcription and translation. In addition, the GO annotations including transport and localization were both over-represented among the differentially expressed genes. The superoxide radical degradation pathway was identified as overrepresented based on the differential expression of the genes GPX7, SOD2, TYRP1, suggesting that some of these animals may be experiencing oxidative stress. GPX7, SOD2 were in lower transcript abundance and TYRP1 was higher in transcript abundance among the low gain-high feed intake animals. The retinoate biosynthesis pathway was also enriched due to the differential expression of the genes AKR1C3, ALDH8A1, RDH8, RDH13, and SDR9C7. These genes were all more highly expressed in the low gain-high intake animals. The pathways identified suggest a role for oxidative stress within the mesenteric fat among the low gain-high intake animals.