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ARS Home » Pacific West Area » Kimberly, Idaho » Northwest Irrigation and Soils Research » Research » Publications at this Location » Publication #326398

Research Project: Improved Sugar Beet Germplasm and Innovative Disease Management Approaches to Increase Yield and Reduce Product Losses

Location: Northwest Irrigation and Soils Research

Title: Beet curly top virus strains associated with sugar beet in Idaho, Oregon, and a survey collection

Author
item Strausbaugh, Carl
item Eujayl, Imad
item Wintermantel, William - Bill

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 2/22/2016
Publication Date: 11/9/2016
Citation: Strausbaugh, C.A., Eujayl, I.A., Wintermantel, W.M. 2016. Beet curly top virus strains associated with sugar beet in Idaho, Oregon, and a survey collection. Phytopathology. 106:S4.108.

Interpretive Summary:

Technical Abstract: Curly top of sugar beet is a serious yield limiting disease in semi-arid production areas caused by Beet curly top virus (BCTV) and vectored by the beet leafhopper. The primary means of control for BCTV is host resistance, but effectiveness of resistance can vary with BCTV strain. BCTV variation was last investigated in Idaho and Oregon during a 2006-2007 survey, but changes in disease severity suggested a need for reevaluation. Therefore, 406 leaf samples symptomatic for curly top were collected from sugar beet plants in commercial sugar beet fields in Idaho and Oregon from 2012 to 2015. DNA was isolated and the BCTV strain composition was investigated based on polymerase chain reaction (PCR) assays with strain specific primers for Severe (Sev) and California/Logan (CA/Logan) strains and nonspecific primers that amplified Worland (Wor)-like strains. The 2006-2007 ID/OR BCTV positive samples from sugar beet included the following strains: 87% Sev, 7% CA/Logan, and 60% Wor-like. BCTV strain distribution in the new survey averaged 2% Sev, 30% CA/Logan, and 87% Wor-like. Whole genome sequencing (GenBank accessions KT276895 to KT276920) with overlapping primers, suggests that the Wor-like strains included Wor, Colorado (CO), and a previously undescribed strain designated Kimberly1 (Kim1). These data indicate there was a shift from Sev being one of the dominant BCTV strains in commercial sugar beet fields in 2006-2007 to being undetected at times in recent years.