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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #325641

Research Project: IDENTIFICATION OF HOST IMMUNE FACTORS AND INTERVENTION STRATEGIES FOR MASTITIS

Location: Ruminant Diseases and Immunology Research

Title: The goat (Capra hircus) mammary gland secretory tissue proteome as influenced by weight loss: A study using label free proteomics

Author
item Hernandez-castellano, Lorenzo - University Of Bern
item Ferreira, Ana - Instituto De Tecnologia Quimica E Biologica
item Nanni, Paolo - University Of Zurich
item Grossmann, Jonas - University Of Zurich
item Arguello, Anastasio - University Of Las Palmas De Gran Canaria
item Capote, Juan - The Canarian Institute Of Agricultural Research
item Cai, Guohong
item Lippolis, John
item Castro, Noemi - University Of Las Palmas De Gran Canaria
item Almeida, Andre - Ross University School Of Veterinary Medicine

Submitted to: Journal of Proteomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/21/2016
Publication Date: 3/18/2016
Publication URL: http://handle.nal.usda.gov/10113/63144
Citation: Hernandez-Castellano, L.E., Ferreira, A.M., Nanni, P., Grossmann, J., Arguello, A., Capote, J., Cai, G., Lippolis, J.D., Castro, N., Almeida, A.M. 2016. The goat (Capra hircus) mammary gland secretory tissue proteome as influenced by weight loss: A study using label free proteomics. Journal of Proteomics. 145:60-69. doi: 10.1016/j.jprot.2016.03.030.

Interpretive Summary: Seasonal weight loss (SWL) is a significant limitation to animal production. Breeds that have evolved in harsh climates have acquired tolerance to SWL through selection. We compared protein expression levels from mammary gland biopsies from two goat breeds, one tolerant and the other susceptible to seasonal weight loss. The breed that is tolerant to seasonal weight loss showed higher expression of immune system related proteins. In contrast, susceptible breed showed higher expression of proteins related to apoptosis. Results indicate that the two goat breeds have a distinct metabolism reaction to SWL, and that proteins related to the immune system and apoptosis could be considered putative candidates as markers of tolerance to SWL.

Technical Abstract: Seasonal weight loss (SWL) is a significant limitation to animal production. Breeds that have evolved in harsh climates have acquired tolerance to SWL through selection. Herein, labelfree proteomics was used to characterize the effects of SWL in two goat breeds with different levels of adaptation to nutritional stress: Majorera (tolerant) and Palmera (susceptible). Ten Majorera and 10 Palmera dairy goats were used, divided in 4 sets, 2 for each breed: underfed and a control group: Majorera Control (MC), Palmera Control (PC), Majorera Restricted (ME) and Palmera Restricted (PE). At day 22, mammary gland biopsy samples were obtained. Label free proteomic analysis enabled the identification of over 1000 proteins, of which 96 showed differential expression between two of the groups within studied comparisons.Majorera breed showed higher expression of immune system related proteins. In contrast, Palmera breed showed higher expression of proteins related to apoptosis. Results indicate that the two goat breeds have a distinct metabolism reaction to SWL, and that proteins related to the immune system and apoptosis such as Cadherin-13, Collagen alpha-1, Nidogen-2, Clusterin and Protein S100-A8 could be considered putative candidates as markers of tolerance to SWL.