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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #323885

Research Project: Genetic Enhancement of Watermelon, Broccoli, and Leafy Brassicas for Economically Important Traits

Location: Vegetable Research

Title: Quantitative trait loci mapping of heat tolerance in a doubled haploid population of broccoli using genotyping-by-sequencing

item Branham, Sandra
item Couillard, David
item Stansell, Zachary
item Farnham, Mark

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2015
Publication Date: 1/11/2016
Citation: Branham, S., Couillard, D.M., Stansell, Z.J., Farnham, M.W. 2016. Quantitative trait loci mapping of heat tolerance in a doubled haploid population of broccoli using genotyping-by-sequencing. Plant and Animal Genome Conference. P0950.

Interpretive Summary: N/A

Technical Abstract: Broccoli is a cool weather vegetable crop with a vernalization requirement to initiate and maintain floral development. Breeding for heat tolerance in broccoli has the potential to both expand viable production areas and extend the growing season. A doubled haploid (DH) population of broccoli (Brassica oleracea var. italica L.) consisting of 159 individuals segregating for heat tolerance was produced by a cross between heat-tolerant and heat-sensitive breeding lines. The population was evaluated for resistance to heat stress (using a 9 point scale) in 3 years of field trials in Charleston, SC. Population tolerance scores displayed a continuous distribution with a mean of 3.4 points and a standard deviation of 1.5 points. Genotyping-by-sequencing followed by imputation with FSFHap yielded 2,335 non-redundant informative single nucleotide polymorphisms (SNPs) for genetic map construction. Composite interval mapping was performed using the quantitiative trait loci (QTL) package in R. Identified QTL and their implications for marker-assisted selection in future breeding efforts will be discussed.