|MAGGIOLI, MAYARA - Iowa State University|
|MCGILL, JODI - Kansas State University|
|VORDERMEIER, MARTIN - Animal & Plant Health Agency Apha|
|BERNEY-MEYERS, LINDA - Albert Einstein College Of Medicine|
|JACOBS JR., WILLIAM - Albert Einstein College Of Medicine|
|LARSEN, MICHELLE - Albert Einstein College Of Medicine|
Submitted to: Clinical and Vaccine Immunology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/10/2015
Publication Date: 12/16/2015
Citation: Waters, W.R., Maggioli, M.F., Palmer, M.V., Thacker, T.C., McGill, J.L., Vordermeier, M., Berney-Meyers, L., Jacobs Jr., W.R., Larsen, M.H. 2015. Interleukin-17A as a biomarker for bovine tuberculosis. Clinical and Vaccine Immunology. 23(2):168-80. doi: 10.1128/CVI.00637-15.
Interpretive Summary: Despite highly successful eradication efforts in several countries, tuberculosis of cattle remains a serious health concern worldwide. In addition, recent outbreaks of tuberculosis in various states demonstrate that the disease is far from eliminated from the United States. Improved techniques are needed for detection of infected cattle as well as improved control strategies (e.g., vaccines). To develop improved tests and vaccines, it is beneficial to first understand the nature of bovine immune responses to the pathogen. In this study, important cell types involved in the immune response of cattle to Mycobacterium bovis infection and vaccination were characterized. This basic information will be useful for development of improved tests and vaccines for cattle.
Technical Abstract: T helper (Th) 17-associated cytokines are integral in the immune response to tuberculosis, initiating both protective and harmful inflammatory responses. The aim of the present study was to evaluate applied aspects of IL-17 biology in the context of Mycobacterium bovis infection of cattle. Using RNA-seq, numerous Th17-associated cytokine genes (including IL-17A, IL-17F, IL-22, IL-19, and IL-27) were up-regulated > 9 fold in response to purified protein derivative stimulation of peripheral blood mononuclear cells from infected cattle. Protective vaccines elicited IL-17A, IL-17F, IL-22, and IL-27 responses. As compared to non-vaccinates, reduced IL-17A responses by vaccinates at 2.5 weeks after M. bovis challenge correlated with reduced disease burden. Additionally, IL-17A and IFN-' responses were highly correlated with similar diagnostic capacity. Present findings support the use of Th17-associated cytokines as biomarkers of infection and protection in the immune response to bovine tuberculosis.