|MAGGIOLI, MAYARA - Iowa State University|
|VORDERMEIER, MARTIN - Animal & Plant Health Agency Apha|
|MCGILL, JODI - Kansas State University|
|LARSEN, MICHELLE - Albert Einstein College Of Medicine|
|JACOBS, WILLIAM - Albert Einstein College Of Medicine|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/25/2015
Publication Date: 11/22/2015
Citation: Maggioli, M.F., Palmer, M.V., Thacker, T.C., Vordermeier, M., Mcgill, J., Larsen, M., Jacobs, W., Waters, W.R. 2015. Polyfunctional cytokine production by central memory T cells from cattle in response to Mycobacterium bovis infection and BCG vaccination. Meeting Abstract. page 33.
Technical Abstract: Polyfunctional T cells simultaneously produce IFN-gamma, IL-2 and TNF-alpha and play relevant roles in several chronic infections, including TB. Mycobacterium bovis infection of cattle elicits ex vivo polyfunctional T cell responses. Vaccine-elicited IFN-gamma Tcm (CD4+ CD45RO+ CCR7+) responses correlate to protection in cattle. The objective was to assess polyfunctional Tcm responses to vaccination with BCG or BCG mutants and subsequent M. bovis challenge in cattle. BCG evoked polyfunctional Tcm to PPD and Ag85A/TB10.4. After challenge, TNF-alpha/IFN-gamma Tcm responses to PPD, Ag85A/TB10.4 or ESAT-6:CFP10 were greater in non-vaccinates in comparison to vaccinates. Both vaccines showed similar protection; however, vaccinated animals that were M. bovis culture negative had lower TNF-alpha/IFN-gamma polyfunctional Tcm responses to ESAT-6:CFP10 3wpi than did culture positive vaccinates. In conclusion, BCG vaccination elicited Tcm polyfunctionality, mainly TNF-alpha/IFN-gamma. Dampened TNF-alpha/IFN-gamma Tcm responses after challenge may indicate enhanced pathogen control and correlate with vaccine elicited protection.