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Research Project: Pathogen Characterization, Host Immune Response and Development of Strategies to Reduce Losses to Disease in Aquaculture

Location: Aquatic Animal Health Research

Title: Hepatic transcriptomic and metabolic responses of hybrid striped bass to acute and chronic hypoxic insult

Author
item Beck, Benjamin
item Fuller, Adam
item Li, Chao - Auburn University
item Green, Bartholomew - Bart
item Rawles, Steven - Steve
item Webster, Carl
item Peatman, Eric - Auburn University

Submitted to: Book of Abstracts World Aquaculture Society
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2015
Publication Date: 2/21/2016
Citation: Beck, B.H., Fuller, S.A., Li, C., Green, B.W., Rawles, S.D., Webster, C.D., Peatman, E. 2016. Hepatic transcriptomic and metabolic responses of hybrid striped bass to acute and chronic hypoxic insult [abstract]. Book of Abstracts World Aquaculture Society Annual Meeting. p. 59.

Interpretive Summary:

Technical Abstract: Striped bass (Morone saxatilis), white bass (Morone chrysops), and their hybrid are an important group of recreational and farmed species in the United States. Regardless of habitat, it is not uncommon for fish of the genus Morone to encounter and cope with conditions of scarce oxygen availability. Previously, we determined that hybrid striped bass reared under conditions of chronic hypoxia exhibited reduced feed intake, lower lipid and nutrient retention, and poor growth. To better understand the molecular mechanisms governing these phenotypes, in the present study, we examined the transcriptomic profiles of hepatic tissue in hybrid striped bass exposed to chronic hypoxia (90 days at 25% oxygen saturation) and acute hypoxia (6 hours at 25% oxygen saturation). Using high-throughput RNA-seq, we found that over 1,400 genes were differentially expressed under disparate oxygen conditions, with the vast majority of transcriptional changes occurring in the acute hypoxia treatment. Gene pathway and bioenergetics analyses revealed hypoxia-mediated perturbation of genes and gene networks related to lipid metabolism, cell death, and changes in hepatic mitochondrial content and cellular respiration. This study offers a more comprehensive view of the temporal and tissue-specific transcriptional changes that occur during hypoxia, and reveal new and shared mechanisms of hypoxia tolerance in teleosts.