|John, Maria - Konkuk University|
|Khan, Farooq - University Of Maryland|
|Luthria, Devanand - Dave|
|Matthews, Benjamin - Ben|
|Natarajan, Savithiry - Savi|
Submitted to: Journal of Food Biochemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/19/2017
Publication Date: 5/16/2017
Citation: John, M., Khan, F., Luthria, D.L., Matthews, B.F., Garrett, W.M., Natarajan, S.S. 2017. proteomic and metabolomic analysis of minimax and Williams 82 soybeans grown under two different conditions. Journal of Food Biochemistry. 41:e12404. https://doi.org/10.1111/jfbc.12404.
DOI: https://doi.org/10.1111/jfbc.12404 Interpretive Summary: Soybean provides an economical source of protein for humans and animals. Different soybean varieties are grown in different geographic locations throughout the United States. These locations have different growing seasons, climates, and different soil compositions. To determine if the natural soybean seed protein and isoflavone profiles vary from cultivar to cultivar and from growing conditions, we characterized soybean seed proteins and isoflavones from two different cultivars (Minimax and Williams 82) in two different conditions (field and greenhouse). We observed significant differences in expression of proteins and amounts of isoflavones between MiniMax and the cultivated soybean, Williams 82, under both growing conditions. These results are important for the evaluation of soybean seed production and will be of interest to soybean growers, breeders, and producers at universities, government agencies, and private industry.
Technical Abstract: Soybeans are an important source of proteins and multiple nutrients for humans and livestock. The objective of this research was to compare the protein (2D-PAGE) and isoflavone (LC-MS) content in a dwarf-sized soybean genotype, MiniMax (Glycine max) and a cultivated normal-sized soybean genotype, Willams 82 (Glycine max). MiniMax is being developed as a model soybean cultivar, because of its small stature and rapid generation time. In addition, we compared the influence of growing conditions (greenhouse versus field) on both protein and isoflavone content in MiniMax and Williams 82. Out of 716 protein spots detected based on the software (Total lab same spots) program, 22 spots were differentially expressed in MiniMax and Williams 82. Some of the differentially expressed proteins identified by mass spectrometry (MALDI-TOF-TOF MS/MS) were seed biotin, ß-conglycinin, glycinin, seed maturation protein, glutathione-S-transferase, and sucrose binding protein. There were varying differences in the expression of 22 proteins for the two genotypes grown under the two environmental conditions. The total yield of quantified isoflavones varied between 0.9 and 3.1mg/g for the two genotypes grown under field and greenhouse conditions. Most isoflavones (>85%) existed in glucoside form. With both genotypes, a higher concentration of isoflavone aglycons was obtained from greenhouse grown samples as compared to the field grown samples. The trend for most isoflavones for the two cultivars grown under field and greenhouse conditions was similar. Both MiniMax and Williams 82 genotypes grown under field conditions showed lower levels of total isoflavones as compared to the samples grown under greenhouse conditions.