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Research Project: Characterization, Etiology, and Disease Management for Vegetable Crops

Location: Vegetable Research

Title: Complete genome sequence of a tomato infecting tomato mottle mosaic virus in New York

Author
item Padmanabhan, Chellappan
item Zheng, Yi - Boyce Thompson Institute
item Li, Rugang
item Fei, Zhangjun - Boyce Thompson Institute
item Ling, Kai-shu

Submitted to: Genome Announcements
Publication Type: Research Notes
Publication Acceptance Date: 11/4/2015
Publication Date: 12/23/2015
Citation: Padmanabhan, C., Zheng, Y., Li, R., Fei, Z., Ling, K. 2015. Complete genome sequence of a tomato infecting tomato mottle mosaic virus in New York. Genome Announcements. 3(6):e01523-15. doi:10.1128/genomeA.01523-15.

Interpretive Summary: Tomato mottle mosaic virus (ToMMV) is an emerging virus, first reported in Mexico in 2013 that has been shown to infect tomato and pepper in Brazil, China, and Iran. In the United State, the virus was first identified on tomatoes in Florida, but its geographic distribution in the U.S. has not been well understood. In a survey of possible viruses infecting experimental plants in up-state New York, using small ribonucleic acid (RNA) deep sequencing technology, ToMMV was identified on Nicotiana benthamiana plants. This is a first report of ToMMV in the northeastern region of the U.S. Such expansion of ToMMV to East Coast states causes concerns for the tomato and pepper industries.

Technical Abstract: Complete genome sequence of an emerging isolate of tomato mottle mosaic virus (ToMMV) infecting experimental nicotianan benthamiana plants in up-state New York was obtained using small RNA deep sequencing. ToMMV_NY-13 shared 99% sequence identity to ToMMV isolates from Mexico and Florida. Broader distribution of this emerging virus caused concerns to the tomato industry. The complete genome sequence of a southern tomato virus (STV) isolate on tomato plants in a seed production field in Bangladesh was obtained for the first time using next generation sequencing. The identified isolate STV_BD-13 shares high degree of sequence identity (99%) with several known STV isolates worldwide.