|ZHENG, YI - Boyce Thompson Institute|
|SHEKASTE-BAND, REZA - University Of Florida|
|HASEGAWA, DANIEL - Boyce Thompson Institute|
|SCOTT, JOHN - University Of Florida|
|FEI, ZHANGJUN - Boyce Thompson Institute|
Submitted to: Tomato Disease Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 9/23/2015
Publication Date: 10/5/2015
Citation: Padmanabhan, C., Zheng, Y., Shekaste-Band, R., Stewart, K.S., Hasegawa, D., Scott, J.W., Fei, Z., Ling, K. 2015. Identification of differentially expressed genes in tomato associated with R-lines Ty-5 against tomato yellow leaf curl virus (TYLCV) and Sw-7 against tomato spotted wilt virus (TSWV). 30th Annual Tomato Disease Workshop. p19.
Interpretive Summary: N/A
Technical Abstract: Tomato yellow leaf curl virus (TYLCV) and tomato spotted wilt virus (TSWV) are two most economically important viruses affecting tomato productions worldwide. Developing a cultivar with resistance to these viruses would be the most effective and economical means of disease management. Comparative analysis on differential gene expression and in functional pathways impacted by a virus infection may lead to the identification of candidate genes for virus resistance. Using two near isogenic breeding lines introgressed between the susceptible and resistance lines (Ty-5 or Sw-7), transcriptome profiling of differentially expressed (DE) genes was conducted using RNA-seq on tomato plants challenge-inoculated with TYLCV (whitefly feeding) or TSWV (mechanical inoculation) at 4, 7, 14, 21, and 35 days post-inoculation (dpi). Although, a number of genes were differentially expressed on tomato plants upon virus infection at various time points, only 10 genes for Ty-5 and 7 genes for Sw-7 were affected at all time points. Most affected functional categories include pathways of immune system, response to stimulus, metabolism, cellular process, biological regulation, catalytic activity, protein binding, cell, membrane, organelle, development, reproduction, and growth. In total, about 137 defense related genes for Ty-5 and 221 genes for TSWV were identified in Ty5 and Sw-7 lines respectively, including NBS-LRR, various transcription factors, endochitinase, chlorophylase 2, cathepsin B-like cysteine proteinase, Beta-1,3-glucanase and others. Some of these genes might facilitate protection against the two important viruses: TYLCV and TSWV. The functional validation and linking DE genes network predictions are in progress.