Submitted to: Fiber Plants: Biology, Biotechnology and Applications
Publication Type: Book / Chapter
Publication Acceptance Date: 9/30/2015
Publication Date: 11/1/2016
Citation: Sakhanokho, H.F., Rajasekaran, K. 2016. Cotton regeneration in vitro. In: Ramawat, K.G., Ahuja, M.R., editors. Fiber Plants: Biology, Biotechnology and Applications. Gewerbestrasse, Switzerland: Springer International Publishing AG. p. 87-110.
Interpretive Summary: Not required
Technical Abstract: H. F. Sakhanokho and K. Rajasekaran Over the years, plant breeders have improved cotton via conventional breeding methods, but these methods are time-consuming. To complement classical breeding and, at times, reduce the time necessary for new cultivar development, breeders have turned to in vitro plant transformation or genetic engineering, relying mostly on two major approaches, Agrobacterium-mediated and particle bombardment transformation techniques. Since its adoption in the 1990s, transgenic technology continues to have a tremendous impact on cotton production not only in the United States but also worldwide. Currently, genetically modified cottons, in particular insect and herbicide tolerant cotton, account for over 90% and 80% of cultivated cotton acreage in the United States and worldwide, respectively. However, efforts in the development of transgenic cotton are hampered by the recalcitrance of most cotton cultivars, particularly the elite cultivars, to regenerate via tissue culture, a step very often necessary for the transformation process. In vitro regeneration of cotton, in particular regeneration via somatic embryogenesis, is highly genotype dependent. In addition, , other factors including explant type, composition and type of media (liquid vs. solid) as well as environmental conditions surrounding the cultures affect the in vitro regeneration of cotton. In this chapter, the current status of different regeneration methods and the factors limiting or enhancing these methods are discussed.