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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #320547

Research Project: Genomic Approaches and Genetic Resources for Improving Rice Yield and Grain Quality

Location: Dale Bumpers National Rice Research Center

Title: Rice varietal differences in arsenite metabolism

Author
item Heuschele, Deborah - Jo
item Pinson, Shannon
item SMITH, AARON - Louisiana State University

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 9/15/2015
Publication Date: 11/17/2015
Citation: Heuschele, D.J., Pinson, S.R., Smith, A.P. 2015. Rice varietal differences in arsenite metabolism. ASA-CSSA-SSSA Annual Meeting Abstracts. Minneapolis, MN, Nov. 15-18, 2015. Poster number: 1108. Paper number: 93248. https://scisoc.confex.com/scisoc/2015am/webprogram/Paper93248.html.

Interpretive Summary:

Technical Abstract: Plants utilize an assortment of survival mechanisms to reduce arsenic toxicity, such as exclusion, translocation, and detoxification. Detoxification is the primary method plants use to mediate mitigate heavy metal stress through formation of reactive oxygen species (ROS) and methylglyoxal (MG) metabolism. Both ascorbic acid (AsA) and glutathione (GSH), compounds generated in response to ROS, increase with application of As in some varieties of rice; this increase has been correlated with the rice variety’s ability to detoxify As. Recently, key enzymes in MG metabolism that produce lactic acid have been shown to be active in the rice genome. The goal of our study was to determine which key components of the heavy metal detoxification pathway are also associated with differences in the amount of arsenic that accumulates in the grain, addressing a potential food safety issue. Six varieties of rice previously determined to produce accumulate especially high or low grain As levels were surveyed for differences in compounds associated with the As-detoxification pathway. We grew these six varieties hydroponically for 14 days and then added As(III) to a subset for 72hrs, growing the other half in standard hydroponic solution. Concentrations of AsA, GSH, D-lactic acid, and As levels were measured in both root and leaf tissue at various time points. GSH and D-lactic acid levels differed between varieties and tissue types. In that these differences coincided with grain As levels among the varieties, the data suggest that As detoxification in the leaves and roots can decrease the amount of As that is transported to and accumulated in rice grains.