Location: Mosquito and Fly ResearchTitle: Identification and transcription profiling of NDUFS8 in Aedes taeniorhynchus (Diptera:Culididae): developmental regulation and environmental response Author
|Zhao, Liming - University Of Florida|
|Kline, Daniel - Dan|
|Allan, Sandra - Sandy|
|Linthicum, Kenneth - Ken|
Submitted to: Open Access Insect Physiology Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/6/2014
Publication Date: 12/18/2014
Publication URL: http://handle.nal.usda.gov/10113/61863
Citation: Zhao, L., Kline, D.L., Becnel, J.J., Chen, J., Allan, S.A., Clark, G.G., Linthicum, K. 2014. Identification and transcription profiling of NDUFS8 in Aedes taeniorhynchus (Diptera:Culididae): developmental regulation and environmental response. Open Access Insect Physiology Journal. 2015(5):1-12. Interpretive Summary: NADH dehydrogenase, located in the inner mitochondrial membrane,is the first enzyme of the mitochondrial electron transport chain that catalyzes the transfer of electrons from NADH to coenzyme Q. NADH dehydrogenase, the largest of the respiratory complexes, is the first enzyme of the mitochondrial electron transport chain. Mitochondrial genes or mitochondrial related genes can be used as genetic markers to identify ecotypes in different populations of plants(1, 2), and animals, including insects and mosquitoes (3-11). The critical role of NADH in the respiratory function of Complex I has been demonstrated by linking mutation in NADH subunits to certain herediatary disease (12). Aedes taeniorhynchus Wiedemann, a nuisance species, has attracted much attention recently (7, 13-18). The purpose of this study was to clone the NADH dehydrogenase -ubiquinone Iron-Sulfate (Fe-S) protein 8 subunit (AetNDUFS8) gene and examine mRNA expression during development and in response to challenge by the pesticide permethrin (19). This information is important for understanding the role of NADH subunits in development and pesticide sensitivity in mosquitoes. Mitochondrially Encoded NADH dehydrogenase subunit 5 (AetNADH5) has been used to identify the species on isolated oceanic islands (7). In present study, AetNADH5 was examined during the development of Ae. taeniorhynchus. Using RNA interference technology to knock down the mitochondrial proteins AetNDUFS8 and AetNADH5 may provide additional targets that can be developed as new pesticides.
Technical Abstract: The cDNA of a NADH dehydrogenase -ubiquinone Fe-S protein 8 subunit (NDUFS8) gene from Aedes (Ochlerotatus) taeniorhynchus Wiedemann has been cloned and sequenced. The full-length mRNA sequence (824 bp) of AetNDUFS8 encodes an open reading region of 651 bp (i.e., 217 amino acids). To detect whether AetNDUFS8 is developmentally regulated, a quantitative real-time polymerase chain reaction (qPCR) were used to examine AetNDUFS8 gene expression levels in different developmental stages of Ae.taeniorhynchus. AetNDUFS8 was expressed at relatively low in egg, larval and pupal stages and highly expressed in the teneral adult stage. qPCR also showed that mRNA transcription levels of AetNDUFS8 were regulated during the time course study in response to permethrin treatment in female Ae. taeniorhynchus. This study suggests that AetNDUFS8 play an important role in the development of Ae. taeniorhynchus and may provide information useful for designing novel strategies for mosquito control.