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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #318638

Research Project: Genetics, Breeding and Reproductive Physiology to Enhance Production of Catfish

Location: Warmwater Aquaculture Research Unit

Title: Complete genome sequence of an Edwardsiella piscicida-like species recovered from tilapia in the United States

item REICHLEY, STEPHEN - Mississippi State University
item Waldbieser, Geoffrey - Geoff
item LAWRENCE, MARK - Mississippi State University
item GRIFFIN, MATT - Mississippi State University

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/24/2015
Publication Date: 9/3/2015
Citation: Reichley, S.R., Waldbieser, G.C., Lawrence, M.L., Griffin, M.J. 2015. Complete genome sequence of an Edwardsiella piscicida-like species recovered from tilapia in the United States. Genome Announcements. 3:e01004-15.

Interpretive Summary: The genus Edwardsiella is comprised of a diverse group of Gram-negative bacteria and, until recently, consisted of only three taxa: E. hoshinae, E. ictaluri, and E. tarda. Recent molecular investigations have led to the proposed resolution of E. tarda into E. tarda, E. piscicida and E. anguillarum. Of these, E. piscicida and E. piscicida-like bacteria demonstrate pathogenicity in fish so development of accurate diagnostic assays is paramount. Toward this goal, a team of scientists from Mississippi State University and the USDA-ARS-Warmwater Aquaculture Research Unit used first- and second- generation DNA sequencing technologies to produce a reference genome sequence assembly of the the LADL05-105 isolate of an E. piscicida-like strain which was initially isolated from cultured tilapia reared in Louisiana. The single chromosome had a length of 4.1 million bases with a predicted 3,686 genes encoding 3,159 proteins and 99 RNAs. This isolate was highly similar (> 99% DNA sequence similarity) to two other E. piscicida-like genomes but distinct from E. piscicida (94% identity), E. ictaluri (92% identity) and E. tarda (84% identity). The genome sequence enables comparisons with other Edwardsiella species to discover genes that are critical for pathogenicity in order to develop diagnostics and therapeutants to reduce catfish production losses.

Technical Abstract: Edwardsiella piscicida-like sp. is a Gram-negative, facultative anaerobe that causes disease in some fish species. In this report we present the complete and annotated genome of isolate LADL05-105, recovered from cultured tilapia reared in Louisiana, which contains a chromosome of 4,142,037 bp and no plasmids.