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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #317250

Research Project: Genetics, Breeding and Reproductive Physiology to Enhance Production of Catfish

Location: Warmwater Aquaculture Research Unit

Title: Complete genome sequence of Edwardsiella tarda (isolate FL95-01)recovered from channel catfish

item REICHLEY, STEPHEN - Mississippi State University
item Waldbieser, Geoffrey - Geoff
item TEKEDAR, HASAN - Mississippi State University
item LAWRENCE, MARK - Mississippi State University
item GRIFFIN, MATT - Mississippi State University

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/21/2015
Publication Date: 6/25/2015
Citation: Reichley, S.R., Waldbieser, G.C., Tekedar, H.C., Lawrence, M., Griffin, M. 2015. Complete genome sequence of Edwardsiella tarda (isolate FL95-01)recovered from channel catfish. Genome Announcements. 3:e00682-15.

Interpretive Summary: The bacteria Edwardsiella tarda has been historically implicated in fish disease outbreaks worldwide, including channel catfish, but this species has recently been divided into two taxons: E. tarda and E. piscicida, which is pathogenic to fish. Several published genomes are actually E. piscicida. A team of scientists from Mississippi State University and the USDA-ARS-Warmwater Aquaculture Research Unit have used new DNA sequencing technologies and sequence assembly algorithms to produce a complete reference genome sequence assembly of the FL95-01 strain of E. tarda. This is the only E. tarda sequence currently available. The genome sequence permits comparative genomic analyses with the E. piscicida isolates that are implicated in fish disease outbreaks. Our ultimate aim is understand virulence and environmental adaptations of these Edwardsiella species in order to develop diagnostics and therapeutants to reduce catfish production losses.

Technical Abstract: Edwardsiella tarda is a Gram-negative facultative anaerobe isolated from fish, reptiles, amphibians, and mammals, including humans. This is a report of the complete and annotated genome of E. tarda isolate FL95-01, recovered from channel catfish (Ictalurus punctatus).