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Title: Detection of novel QTLs for foxglove aphid resistance in soybean

item LEE, JU SEOK - Dankook University
item YOO, MIN-HO - Dankook University
item JUNG, JIN KYO - National Institute Of Crop Science - Korea
item Bilyeu, Kristin
item LEE, JEONG-DONG - Kyungpook National University
item KANG, SUNGTAEG - Dankook University

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/11/2015
Publication Date: 8/1/2015
Publication URL:
Citation: Lee, J., Yoo, M., Jung, J., Bilyeu, K.D., Lee, J., Kang, S. 2015. Detection of novel QTLs for foxglove aphid resistance in soybean. Theoretical and Applied Genetics. 128(8):1481-1488.

Interpretive Summary: Soybean yields are negatively impacted by insect pests. Having a toolbox of knowledge and sources of resistance to different pests is important for effective defense against emerging insect issues.In this work we determined that a wild soybean accession had resistance to the foxglove aphid pest, and we identified a region of the genome that contained a major effect gene for resistance to the pest. This source of resistance to the aphid is novel, but, intriguingly, it maps to the same chromosome region as a different gene for soybean aphid resistance. The identification of molecular markers linked to the foxglove aphid resistance region can be immediately applied in soybean breeding efforts. Understanding the overlap and independence in resistance mechanisms may help find more generalized ways to combat soybean insect pests.

Technical Abstract: Foxglove aphid, Aulacorthum solani (Kaltenbach), is a Hemipteran insect that infected a wide variety of plants worldwide and caused serious yield losses in crops. The objective of this study was to identify the putative QTL for foxglove aphid resistance in wild soybean, PI 366121, (Glycine soja Sieb. and Zucc.). One hundred and forty one F2-derived F8 recombinant inbred lines developed from a cross of susceptible Williams 82 and PI 366121, were used. The phenotyping of antibiosis and antixenosis was done through choice and no-choice assays with total plant damage (TPD) and primary infestation leaf damage (PLD); a genome-wide molecular linkage map was constructed with 504 single nucleotide polymorphism markers utilizing a GoldenGate assay. Using inclusive composite interval mapping analysis for foxglove aphid resistance, one major candidate QTL on chromosome 7 and 3 minor QTL regions on chromosome 3, 6 and 18 were identified. The major QTL on chromosome 7 showed both antixenosis and antibiosis resistance responses. However, the minor QTLs showed only antixenosis resistance response. The major QTL mapped to a different chromosome than the previously identified foxglove aphid resistance QTL, Raso1, from the cultivar Adams. Also, the responses to the Korea biotype foxglove aphid were different for Raso1, and the gene from PI 366121 against the Korea biotype foxglove aphid were different. Thus the foxglove aphid resistance gene from PI 366121 was determined to be an independent gene to Raso1 and designated to Raso2. This result could be useful in breeding for new foxglove aphid resistant soybean cultivars.