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Research Project: Genetic Enhancement of Watermelon, Broccoli, and Leafy Brassicas for Economically Important Traits

Location: Vegetable Research

Title: A major quantitative trait locus is associated with Fusarium Wilt Race 1 resistance in watermelon

Author
item Lambel, S - Harris Moran Seed Company
item Lanini, B - Harris Moran Seed Company
item Vivoda, E - Harris Moran Seed Company
item Fauve, J - Harris Moran Seed Company
item Wechter, William - Pat
item Harris-shultz, Karen
item Massey, Laura
item Levi, Amnon

Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 7/13/2014
Publication Date: 8/8/2014
Citation: Lambel, S., Lanini, B., Vivoda, E., Fauve, J., Wechter, W.P., Harris-Shultz, K.R., Massey, L.M., Levi, A. 2014. A major quantitative trait locus is associated with Fusarium Wilt Race 1 resistance in watermelon. Annual International Plant & Animal Genome Conference. 127:2105-2115.

Interpretive Summary: N/A

Technical Abstract: Fusarium wilt is a major disease of watermelon caused by the soil-borne fungus Fusarium oxysporum Schlechtend.:Fr. f. sp. niveum (E.F. Sm.) W.C. Snyder & H.N. Hans (Fon). A genetic population of 186 F3 families (24 plants in each family) exhibited continuous segregation for Fon race 1 response. Genotyping-by-sequencing (GBS) technology was used on these 91 selected F2 samples to produce 266 single nucleotide polymorphism (SNP) markers, representing the 11 chromosomes of watermelon. A major quantitative trait locus (QTL) associated with resistance to Fon race 1 was identified with a peak logarithm of odds (LOD) of 33.31 and 1-LOD confidence interval from 2.3 - 8.4 cM on chromosome 1 of the watermelon genetic map. This QTL is positioned in a genomic region where several disease-resistance candidate genes, known as pathogenesis-related (PR) protein gene sequences were identified. Additional independent, but minor QTLs were identified on chromosome 1 (LOD 4.16), chromosome 3 (LOD 4.36), chromosome 4 (LOD 4.52), chromosome 9 (LOD 6.8), and chromosome 10 (LOD 5.03 and 4.26). The results in this study are useful for the identification of resistance genes and elucidating resistance to Fusarium wilt in watermelon.