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Title: Tissue distribution of HSPA9/mortalin in avian species and its regulation by gender, genotype and heat stress

item NGUYEN, PHUONG - University Of Arkansas
item GREENE, ELISABETH - University Of Arkansas
item Huff, Geraldine
item PIEKARSKI, ALISSA - University Of Arkansas
item Donoghue, Ann - Annie
item ANTHONY, NICHOLAS - University Of Arkansas
item BOTJE, WALTER - University Of Arkansas
item DRIDI, SAMI - University Of Arkansas

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2014
Publication Date: 1/26/2015
Citation: Nguyen, P.H., Greene, E.S., Huff, G.R., Piekarski, A., Donoghue, A.M., Anthony, N.B., Botje, W.G., Dridi, S. 2015. Tissue distribution of HSPA9/mortalin in avian species and its regulation by gender, genotype and heat stress. [abstract]. Poult. Sci. 94:17 (E-Suppl. 1).

Interpretive Summary:

Technical Abstract: Heat shock 70kDa protein 9 (HSPA9)/mortalin is a multipotent chaperone regulating cellular processes ranging from stress response to energy homeostasis. HSPA9 has been extensively studied in mammals however there is a paucity of information in avian species. The present study aimed to characterize HSPA9 gene in chicken (Gallus gallus) and Japanese quail (Coturnix japonica) and to determine its regulation by gender, genotype and heat stress. Using Polymerase Chain Reaction (PCR), we found that HSPA9 is ubiquitously expressed in both chicken and quail. The chicken HSPA9 sequence had 66.6, 69.7, 70, 71.6 and 78% homology with mouse, human, rat, porcine, and bovine HSPA9 sequences, respectively. Phylogenetic analysis indicates that chicken HSPA9 is more closely related to human and rodent orthologs. Quantitative analysis using real-time PCR showed that in female Jungle Fowl the highest amount of HSPA9 was found in the ovary followed by the intestine, brain, heart, liver, leg muscle and gizzard. In male, however, the expression of HSPA9 was higher in the adipose tissue, followed by lung, kidney, intestine, heart, and leg muscle. Interestingly, when we profile the two genders together, female chicken exhibited high expression of HSPA9 in the brain and the ovary compared to male. However male chickens displayed higher HSPA9 expression in the adipose tissue, the lung and the kidney compared to female. In order to assess whether the expression of HSPA9 is affected by genotype and genetic selection, two male quail lines were used. These two lines were established by long-term divergent selection for circulating corticosterone response to restraint stress, after which the low stress line (resistant, R) had 66% low circulating corticosterone levels compared to their high stress (sensitive, S) counterpart. The S line displayed higher HSPA9 mRNA levels in heart, gizzard and testis compared to R line. R line, however, exhibited higher HSPA9 expression in liver, lung and kidney compared to S line. Importantly, acute heat stress differently regulates hepatic HSPA9 gene expression with down regulation in R line and up regulation in S line. In conclusion, this is the first report, to our knowledge, that describes the tissue distribution and the regulation of HSPA9 in avian species. Interestingly, the HSPA9 expression seems to be line dependent in S and R quail indicating that HSPA9 may be a key differential molecular signature in stress response in these lines.