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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Nutrition and Environmental Management Research » Research » Publications at this Location » Publication #311929

Research Project: Improved Nutrient Efficiency of Beef Cattle and Swine

Location: Nutrition and Environmental Management Research

Title: Differential expression of genes in the jejunum of steers with feed efficiency phenotypes

Author
item BUTLER, ASHLEY - North Carolina State University
item Lindholm-Perry, Amanda
item Freetly, Harvey

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 12/11/2014
Publication Date: 3/1/2015
Citation: Butler, A.R., Lindholm-Perry, A.K., Freetly, H.C. 2015. Differential expression of genes in the jejunum of steers with feed efficiency phenotypes [abstract]. Journal of Animal Science. 93(Supplement 2):179.

Interpretive Summary:

Technical Abstract: The small intestine is an important site of digestion and absorption of nutrients in cattle, and has the potential to significantly impact feed efficiency. We hypothesized that the differences in feed efficiency phenotypes of beef cattle can be partially explained by the differences in gene expression in the small intestine. The objective of this study was to determine whether cattle feed intake or growth phenotypes are related to transcript abundance of genes expressed in the small intestine of steers (n=16) with extreme phenotypes. The jejunum was the focus of this particular study. Individual DM intake (DMI) and BW gain were measured on 248 steers for 84 d. BW gain was regressed on DMI and steers were assigned to 4 Cartesian quadrants. The four most extreme steers from each quadrant were identified and selected for jejunum sampling and analysis. RNA was extracted from the jejunum samples and the RNA samples were quantified and analyzed for integrity. The difference in RNA transcript abundance between the groups was measured using the Affymetrix GeneAtlas Microarray System with the Bovine Gene 1.1 ST Array Strips. The data were analyzed using the Affymetrix Transcriptome Analysis Console. Genes differentially expressed in steers with high intake/high gain phenotypes were in networks involved with lipid metabolism and small molecule biochemistry (CYSTM1, CRYAB, EDIL3). Genes with expression unique to animals with low intake/high gain phenotypes were related to proteolysis and hydrolase functions (USP18, MMP14, LYZ). Genes uniquely expressed in animals with low intake/low gain animals were related to stress responses (HSPH1, HSPA6, DNAJB1) and genes differentially expressed in high intake/low gain animals were involved in cell-mediated immune response (IL1RN, CCL8). These results suggest that the differential expression of genes in the jejunum of steers may affect feed efficiency.