Location: Healthy Body Weight ResearchTitle: Endocrine modulators of mouse subcutaneous adipose tissue beige adipocyte markers Author
Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 1/15/2015
Publication Date: 3/28/2015
Citation: Garcia Garcia, R.A., Claycombe, K.J., Roemmich, J.N. 2015. Endocrine modulators of mouse subcutaneous adipose tissue beige adipocyte markers [abstract]. Journal of Federation of American Societies for Experimental Biology. 29:595.5. Interpretive Summary:
Technical Abstract: The stromal vascular fraction (SVF) of subcutaneous adipose tissue contains precursors that can give rise to beige adipocytes. Beige adipocytes are characterized by the expression of specific markers, but it is not clear which markers best evaluate beige adipocyte differentiation. Both regulators of ß-adrenoceptor and PPAR-gamma mediated pathways modulate energy expenditure and beige adipocyte differentiation. However, it is unclear whether PPAR-gamma agonists (e.g., rosiglitazone) and adrenergic agonists (e.g., isoproterenol) have additive effects on expression of beige adipocyte markers. Thus, we isolated SVF cells from subcutaneous white adipose tissue of male C57Bl/6J mice, differentiated these cells into beige adipocytes with rosiglitazone, and evaluated the effect of isoproterenol on beige cell markers such as CD137, P2RX5, and CITED1. These 3 markers were increased 6-fold or more in beige cells, suggesting that they can be used to identify these adipocytes in the unstimulated state. CD137 and P2RX5 maintained their specificity after adrenergic stimulation as they were increased and decreased, respectively, in beige and non-beige cells. CITED1 was increased a large amount by adrenergic stimulation in non-beige cells, and thus loss its specificity. Other proposed beige markers such as EBF2 and PAT2 were increased in beige cells less than 2-fold even after adrenergic stimulation, whereas TMEM26 was 50% lower in beige cells and unchanged by adrenergic stimulation. Our results indicate there is significant heterogeneity among proposed beige cells markers in their ability to identify these cells as well as significant interactions among these markers and ß-adrenoceptor mediated pathways. Supported by USDA/ARS CRIS project No. 3062-51000-052-00D.