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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #311336

Title: Effect of skin test on serum antibody responses to Mycobacterium bovis infection in cattle

item Waters, Wade
item NELSON, JEFF - Animal And Plant Health Inspection Service (APHIS)
item Thacker, Tyler
item MAGGIOLI, MAYARA - Iowa State University
item STAFNE, MOLLY - Iowa State University
item BASS, KRISTIN - Bethyl Laboratories, Inc
item LINSCOTT, RICK - Idexx Laboratories
item LAWRENCE, JOHN - Idexx Laboratories
item Palmer, Mitchell

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/6/2014
Publication Date: 11/6/2014
Citation: Waters, W.R., Nelson, J., Thacker, T.C., Maggioli, M., Stafne, M., Bass, K., Linscott, R., Lawrence, J., Palmer, M.V. 2014. Effect of skin test on serum antibody responses to Mycobacterium bovis infection in cattle [abstract]. United States Animal Health Association. Abstract No. 22.

Interpretive Summary:

Technical Abstract: Recently, several serologic tests designed to detect immunodominant antibodies to M. bovis antigens (e.g., MPB83, MPB70, ESAT-6, and CFP10) have emerged for potential use with samples from cattle. Of these, a commercial ELISA to MPB83/MPB70 (M. bovis antibody ELISA) has gained approval for use in cattle for bovine tuberculosis control programs by the Office International des Epizooties and United States Department of Agriculture. In the present study, the effect of injection of purified protein derivatives (PPD) for caudal fold (CFT) and comparative cervical (CCT) skin tests on serum antibody responses were evaluated with samples from cattle experimentally-infected with M. bovis (n = 8, aerosol challenge). Injection of M. bovis PPD for CFT (89 days after aerosol challenge) elicited serum antibody responses detectable within 1 week by the IDEXX M. bovis antibody ELISA. Positive responses were detectable in all animals up to 74 days after PPD administration. Injection of M. avium and M. bovis PPDs for CCT (105 days after CFT) resulted in a dramatic increase in antibody responses in all animals. Antibody avidity, as measured by an ammonium thiocyanate assay, also increased upon injection of PPDs for CCT. These findings demonstrate that the anamnestic response elicited by injection of PPD(s) for skin test results in both qualitative and quantitative increases in serum antibody responses in M. bovis-infected cattle, of diagnostic relevance.