Submitted to: Plant Dormancy Symposium
Publication Type: Book / Chapter
Publication Acceptance Date: 11/10/2014
Publication Date: 4/14/2015
Citation: Dogramaci, M., Horvath, D.P., Anderson, J.V. 2015. Meta-analysis identifies potential molecular markers for endodormancy in crown buds of leafy spurge; a herbaceous perennial. In: Anderson, J.V., editor. Advances in Plant Dormancy. Cham, Switzerland. Springer International Publishing AG. pp.197-219.
Interpretive Summary: Vegetative shoot growth originating from underground vegetative buds of perennial weeds, such as leafy spurge, is critical for survival after episodes of severe stress. Although leafy spurge is considered an invasive weed in North American ecosystems, it has found value as a model for examining how environmental factors influence molecular processes associated with well-defined phases of dormancy in underground vegetative buds. Inhibition of new aerial shoot growth from these underground vegetative buds coincides with autumn-induced death of aerial tissue, even when environmental conditions can still be conducive for growth. Analysis of expression data from multiple studies identified five transcripts that may be potential molecular markers for inhibition of shoot growth from underground vegetative buds of leafy spurge. Characterization of DNA sequence from several of these genes identified conserved regulatory elements that might serve as potential targets for manipulating plant growth and development.
Technical Abstract: Vegetative shoot growth originating from underground adventitious buds (UABs) of herbaceous perennials such as leafy spurge (Euphorbia esula L.) is critical for survival after episodes of severe abiotic stress. Although leafy spurge is considered an invasive weed in North American ecosystems, it has found value as a model for examining how environmental factors influence molecular processes associated with well-defined phases of dormancy in UABs. A transition from para- to endo-dormancy in UABs of leafy spurge coincides with autumn-induced senescence of the aerial tissue, which is critical for inhibiting initiation of new vegetative shoots when environmental conditions are still conducive for growth. Meta-analysis of global transcriptome data identified transcripts most similar to Arabidopsis COP1, HY5, MAF3, RD22, and RVE1 as potential molecular markers for endodormancy in UABs of leafy spurge. Characterization of the upstream promoter regions for several of these leafy spurge genes identified conserved ABRE-, ARR1-, and MYC-cis-acting regulatory elements. Based on these results, we further refine existing hypothetical models that incorporate proposed regulatory mechanisms for putative leafy spurge MADS-Box domain (MAF3)- and Myb-like (RVE1-like)-transcription factors in endodormant UABs.