|CHEN, JOHNATHAN - University Of Texas|
|SUN, LIANGFENG - University Of Texas|
|Edwards, Judson - Vince|
Submitted to: Bioceramics Development and Applications
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/27/2014
Publication Date: 7/4/2014
Citation: Chen, J.Y., Sun, L., Edwards, J.V. 2014. Regenerated cellulose fiber and film immobilized with lysozyme. Bioceramics Development and Applications. 4(078):1-5. doi:10.4172/2090-5025.100078.
Interpretive Summary: The creation of new markets for value-added, protective cotton textiles is currently an agriculture issue. Work in the Southern Regional Research Center’s Cotton Chemistry Utilization Unit is addressing the application of bio-active molecules to cotton textiles as a model to explore the creation of new value-added cotton products with highly selective properties and environmentally acceptable finishes. As an example of a protein’s biological activity on cotton, enzyme conjugates of cellulose on cotton performance fabrics have been studied for their antimicrobial activity. In this paper we explore the attachment of lysozyme to extruded cotton. The cotton is solubilized in an ionic solvent and reacted with lysozyme followed by extrusion into microbfibers. This approach may have applications to implantables useful in tissue engineering, and forms the basis for a potential National Institutes of Health grant application for use of innovative materials in tissue engineering.
Technical Abstract: The present work reports an initial engineering approach for fabricating lysozyme-bound regenerated cellulose fiber and film. Glycine-esterified cotton was dissolved in an ionic liquid solvent 1–Butyl–3–methylimidazolium Chloride (BMIMCl) in which lysozyme was activated and covalently attached to cotton cellulose through an enzymatic conjugation between its carboxyl groups and glycine cellulose’s amino groups. The resulting solution was extruded for fiber/film formation in a water bath. After performing a bicinchoninic acid (BCA) protein assay, quantity of attached lysozyme to cellulose fiber/film was evaluated. The study exhibited that a synthesis of lysozyme conjugation on cellulose in BMIMCl could be completed in a control manor, resulting in a cellulose solution suitable for fiber/film production. It was also found that lysozyme could be successfully immobilized onto the cellulose fiber and film regenerated from solution spinning with a reasonable amount ranging from 197.6 to 343.7 µg/mL'mg.